Overlaid transcriptional and proteome analyses identify mitotic kinesins as important targets of arylsulfonamide-mediated RBM39 degradation Journal Article

   

Authors:	Coomar, S.; Mota, P.; Penson, A.; Schwaller, J.; Abdel-Wahab, O.; Gillingham, D.
Article Title:	Overlaid transcriptional and proteome analyses identify mitotic kinesins as important targets of arylsulfonamide-mediated RBM39 degradation
Abstract:	Certain arylsulfonamides (ArSulf) induce an interaction between the E3 ligase substrate adaptor DCAF15 and the critical splicing factor RBM39, ultimately causing its degradation. However, degradation of a splicing factor introduces complex pleiotropic effects that are difficult to untangle, since, aside from direct protein degradation, downstream transcriptional effects also influence the proteome. By overlaying transcriptional data and proteome datasets, we distinguish transcriptional from direct degradation effects, pinpointing those proteins most impacted by splicing changes. Using our workflow, we identify and validate the upregulation of the arginine-and-serine rich protein (RSRP1) and the downregulation of the key kinesin motor proteins KIF20A and KIF20B due to altered splicing in the absence of RBM39. We further show that kinesin downregulation is connected to the multinucleation phenotype observed upon RBM39 depletion by ArSulfs. Our approach should be helpful in the assessment of potential cancer drug candidates which target splicing factors. Implications: Our approach provides a workflow for identifying and studying the most strongly modulated proteins when splicing is altered. The work also uncovers a splicing-based approach toward pharmacologic targeting of mitotic kinesins. © 2023 The Authors; Published by the American Association for Cancer Research.
Journal Title:	Molecular Cancer Research
Volume:	21
Issue:	8
ISSN:	1541-7786
Publisher:	American Association for Cancer Research
Date Published:	2023-08-01
Start Page:	768
End Page:	778
Language:	English
DOI:	10.1158/1541-7786.Mcr-22-0541
PUBMED:	37171980
PROVIDER:	scopus
PMCID:	PMC10395616
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85166939846&doi=10.1158%2f1541-7786.MCR-22-0541&partnerID=40&md5=3013d5985ac72ee94140752f48dda748
Notes:	Article -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/208797