Use of native-PAGE for the identification of epichaperomes in cell lines Journal Article
| Authors: | Roychowdhury, T.; Santhaseela, A. R.; Sharma, S.; Panchal, P.; Rodina, A.; Chiosis, G.; Calderwood, S. K.; Prince, T. L. |
| Editors: | Calderwood, S. K.; Prince, T. L. |
| Article Title: | Use of native-PAGE for the identification of epichaperomes in cell lines |
| Abstract: | Epichaperomes are disease-associated pathologic scaffolds, composed of tightly bound chaperones, co-chaperones, and other factors. They mediate anomalous protein–protein interactions inside cells, which aberrantly affects the function of protein networks, and in turn, cellular phenotypes. Epichaperome study necessitates the implementation of methods that retain these protein complexes in their native cellular states for analysis. Here we describe a protocol for detection and composition analysis of epichaperomes in cell homogenates through native polyacrylamide gel electrophoresis. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | cell line; electrophoresis, gel, two-dimensional; hsp70; two dimensional gel electrophoresis; polyacrylamide gel electrophoresis; electrophoresis, polyacrylamide gel; chaperone; molecular chaperones; oligomers; procedures; chaperones; heat shock protein 90 (hsp90); epichaperomes; high-order assemblies; immunoblot; multimolecular protein complexes; native polyacrylamide gel electrophoresis (native-page); non-denaturing gel; native polyacrylamide gel electrophoresis |
| Journal Title: | Chaperones: Methods and Protocols. 2nd ed |
| Volume: | 2693 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2023-01-01 |
| Start Page: | 175 |
| End Page: | 191 |
| Language: | English |
| DOI: | 10.1007/978-1-0716-3342-7_14 |
| PROVIDER: | scopus |
| PMCID: | PMC10448758 |
| PUBMED: | 37540435 |
| DOI/URL: | |
| Notes: | Book chapter: 14 -- This chapter is part of a volume (ISBN: 978-1-0716-3341-0) that is included in a series titled "Methods in Molecular Biology" -- The MSK Cancer Center Support Grant (P30 CA008748) is listed the chapter acknowledgements -- Source: Scopus |
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