Modeling sarcoma relevant translocations using CRISPR-Cas9 in human embryonic stem derived mesenchymal precursors Journal Article

   


Authors: Vanoli, F.; Antonescu, C. R.
Article Title: Modeling sarcoma relevant translocations using CRISPR-Cas9 in human embryonic stem derived mesenchymal precursors
Abstract: The role of cancer relevant translocations in tumorigenesis has been historically hampered by the lack of faithful in vitro and in vivo models. The development of the latest genome editing tools (e.g., CRISPR-Cas9) allowed modeling of various chromosomal translocations with different effects on proliferation and transformation capacity depending on the cell line used and secondary genetic alterations. The cellular context is particularly relevant in the case of oncogenic fusions expressed in sarcomas whose histogenesis remain uncertain. Moreover, recent studies have emphasized the increased frequency of gene fusion promiscuity across different mesenchymal tumor entities, which are clinicopathologically unrelated. This review provides a summary of different strategies utilized to generate cancer models with a focus on fusion-driven mesenchymal neoplasia. © 2023 Wiley Periodicals LLC.
Keywords: gene translocation; genetics; sarcoma; gene rearrangement; translocation, genetic; translocations; human embryonic stem cells; humans; human; crispr cas system; crispr-cas9; gene editing; crispr-cas systems; mesenchymal precursor cells
Journal Title: Genes Chromosomes and Cancer
Volume: 62
Issue: 9
ISSN: 1045-2257
Publisher: Wiley Periodicals, Inc  
Date Published: 2023-09-01
Start Page: 501
End Page: 509
Language: English
DOI: 10.1002/gcc.23141
PUBMED: 36965130
PROVIDER: scopus
PMCID: PMC10725040
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151961266&doi=10.1002%2fgcc.23141&partnerID=40&md5=9854eadcc7aea95de0a124a88b39de8b
Notes: Review -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Fabio Vanoli -- Source: Scopus
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MSK Authors
  1. Cristina R Antonescu
    895 Antonescu
  2. Fabio Vanoli
    22 Vanoli
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