Tracking bispecific antibody-induced T cell trafficking using luciferase-transduced human T cells Journal Article
| Authors: | Espinosa-Cotton, M.; Guo, H. F.; Cheung, N. K. V. |
| Article Title: | Tracking bispecific antibody-induced T cell trafficking using luciferase-transduced human T cells |
| Abstract: | T cell-engaging bispecific antibodies (T-BsAbs) are in various stages of preclinical development and clinical testing for solid tumors. Factors such as valency, spatial arrangement, interdomain distance, and Fc mutations affect the anti-tumor efficacy of these therapies, commonly by influencing the homing of T cells to tumors, which remains a major challenge. Here, we describe a method to transduce activated human T cells with luciferase, allowing in vivo tracking of T cells during T-BsAb therapy studies. The ability of T-BsAbs to redirect T cells to tumors can be quantitatively evaluated at multiple time points during treatment, allowing researchers to correlate the anti-tumor efficacy of T-BsAbs and other interventions with the persistence of T cells in tumors. This method alleviates the need to sacrifice animals during treatment to histologically assess T cell infiltration and can be repeated at multiple time points to determine the kinetics of T cell trafficking during and after treatment. |
| Keywords: | neoplasm; neoplasms; t lymphocyte; t-lymphocytes; animal; animals; antibodies, bispecific; bispecific antibody; humans; human |
| Journal Title: | Journal of Visualized Experiments |
| Issue: | 195 |
| ISSN: | 1940-087X |
| Publisher: | MYJoVE Corporation |
| Date Published: | 2023-05-01 |
| Start Page: | e64390 |
| Language: | English |
| DOI: | 10.3791/64390 |
| PUBMED: | 37246883 |
| PROVIDER: | scopus |
| PMCID: | PMC10999115 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work