High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline Journal Article
| Authors: | Wang, G.; Lyudovyk, O.; Kim, J. Y.; Lin, Y. H.; Elhanati, Y.; Mathew, D.; Wherry, E. J.; Herati, R. S.; Greenplate, A. R.; Greenbaum, B.; Vardhana, S. A.; Huang, A. C. |
| Article Title: | High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline |
| Abstract: | The current abundance of immunotherapy clinical trials presents an opportunity to learn about the underlying mechanisms and pharmacodynamic effects of novel drugs on the human immune system. Here, we present a protocol to study how these immune responses impact clinical outcomes using large-scale high-throughput immune profiling of clinical cohorts. We describe the Human Immune Profiling Pipeline, which comprises an end-to-end solution from flow cytometry results to computational approaches and unsupervised patient clustering based on lymphocyte landscape. For complete details on the use and execution of this protocol, please refer to Lyudovyk et al. (2022).1 © 2023 The Authors |
| Keywords: | immunology; health sciences; flow cytometry/mass cytometry |
| Journal Title: | STAR Protocols |
| Volume: | 4 |
| Issue: | 2 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2023-06-16 |
| Start Page: | 102289 |
| Language: | English |
| DOI: | 10.1016/j.xpro.2023.102289 |
| PROVIDER: | scopus |
| PMCID: | PMC10193120 |
| PUBMED: | 37159385 |
| DOI/URL: | |
| Notes: | Article -- MSK corresponding authors are Benjamin Greenbaum and Santosha Vardhana -- Export Date: 1 June 2023 -- Source: Scopus |
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