Endovascular image-guided sampling of tumor-draining veins provides an enriched source of oncological biomarkers Journal Article
| Authors: | Tamrazi, A.; Sundaresan, S.; Gulati, A.; Tan, F. J.; Wadhwa, V.; Bartlett, B. R.; Diaz, L. A. Jr |
| Article Title: | Endovascular image-guided sampling of tumor-draining veins provides an enriched source of oncological biomarkers |
| Abstract: | Introduction: Circulating tumor-derived biomarkers can potentially impact cancer management throughout the continuum of care. This small exploratory study aimed to assess the relative levels of such biomarkers in the tumor-draining vascular beds in patients with solid tumors compared to levels in their peripheral veins. Methods: Using an endovascular image-guided approach, we obtained blood samples from peripheral veins and other vascular compartments–including the most proximal venous drainage from solid tumors–from a set of nine oncology patients with various primary and metastatic malignancies. We then interrogated these samples for a panel of oncological biomarkers, including circulating tumor cells (CTCs), exosome-derived microRNAs (miRNAs), circulating tumor DNA (ctDNA) mutations, and certain cancer-related proteins/biochemical markers. Results: We found substantially higher levels of CTCs, certain miRNAs, and specific ctDNA mutations in samples from vascular beds closer to the tumor compared with those from peripheral veins and also noted that some of these signals were altered by treatment procedures. Discussion: Our results indicate that tumor-proximal venous samples are highly enriched for some oncological biomarkers and may allow for more robust molecular analysis than peripheral vein samples. Copyright © 2023 Tamrazi, Sundaresan, Gulati, Tan, Wadhwa, Bartlett and Diaz. |
| Keywords: | adult; clinical article; aged; middle aged; iodinated poppyseed oil; chemoembolization; flow cytometry; hysterectomy; biological marker; computer assisted tomography; microrna; oncology; nephrectomy; fluorescence in situ hybridization; blood sampling; real time polymerase chain reaction; cryoablation; minimally invasive; abdominal hysterectomy; circulating tumor cell; celiac artery; transmission electron microscopy; iliac vein; exosome; rna isolation; vascular access; percutaneous transluminal angioplasty; transjugular intrahepatic portosystemic shunt; high throughput sequencing; ultracentrifugation; portosystemic anastomosis; human; article; circulating tumor dna; peripheral vein; whole exome sequencing; liquid biopsy; circulating tumor cell (ctc); circulating tumor dna (ctdna); endovascular surgery; microrna (mirna); oncological biomarker; tumor-draining vein; tumor-proximal |
| Journal Title: | Frontiers in Oncology |
| Volume: | 13 |
| ISSN: | 2234-943X |
| Publisher: | Frontiers Media S.A. |
| Date Published: | 2023-03-17 |
| Start Page: | 916196 |
| Language: | English |
| DOI: | 10.3389/fonc.2023.916196 |
| PROVIDER: | scopus |
| PMCID: | PMC10064007 |
| PUBMED: | 37007151 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 May 2023 -- Source: Scopus |
