Contribution of mutant HSC clones to immature and mature cells in MDS and CMML, and variations with AZA therapy Journal Article
| Authors: | Schnegg-Kaufmann, A. S.; Thoms, J. A. I.; Bhuyan, G. S.; Hampton, H. R.; Vaughan, L.; Rutherford, K.; Kakadia, P. M.; Lee, H. M.; Johansson, E. M. V.; Failes, T. W.; Arndt, G. M.; Koval, J.; Lindeman, R.; Warburton, P.; Rodriguez-Meira, A.; Mead, A. J.; Unnikrishnan, A.; Davidson, S.; Polizzotto, M. N.; Hertzberg, M.; Papaemmanuil, E.; Bohlander, S. K.; Faridani, O. R.; Jolly, C. J.; Zanini, F.; Pimanda, J. E. |
| Article Title: | Contribution of mutant HSC clones to immature and mature cells in MDS and CMML, and variations with AZA therapy |
| Abstract: | Myelodysplastic neoplasms (MDSs) and chronic myelomonocytic leukemia (CMML) are clonal disorders driven by progressively acquired somatic mutations in hematopoietic stem cells (HSCs). Hypomethylating agents (HMAs) can modify the clinical course of MDS and CMML. Clinical improvement does not require eradication of mutated cells and may be related to improved differentiation capacity of mutated HSCs. However, in patients with established disease it is unclear whether (1) HSCs with multiple mutations progress through differentiation with comparable frequency to their less mutated counterparts or (2) improvements in peripheral blood counts following HMA therapy are driven by residual wild-type HSCs or by clones with particular combinations of mutations. To address these questions, the somatic mutations of individual stem cells, progenitors (common myeloid progenitors, granulocyte monocyte progenitors, and megakaryocyte erythroid progenitors), and matched circulating hematopoietic cells (monocytes, neutrophils, and naïve B cells) in MDS and CMML were characterized via high-throughput single-cell genotyping, followed by bulk analysis in immature and mature cells before and after AZA treatment. The mutational burden was similar throughout differentiation, with even the most mutated stem and progenitor clones maintaining their capacity to differentiate to mature cell types in vivo. Increased contributions from productive mutant progenitors appear to underlie improved hematopoiesis in MDS following HMA therapy. © 2023 The American Society of Hematology |
| Keywords: | controlled study; treatment response; chronic myelomonocytic leukemia; human cell; somatic mutation; genetics; leukemia, myelomonocytic, chronic; metabolism; multiple cycle treatment; thrombocyte; cell maturation; erythroid precursor cell; hemoglobin; in vivo study; cell differentiation; b lymphocyte; myelodysplastic syndrome; neutrophil; hematopoietic stem cells; hematopoiesis; erythrocyte; hematopoietic stem cell; heterozygosity loss; cell clone; monocyte; monocytes; lymphoid progenitor cell; megakaryocyte; clone cells; myeloid progenitor cell; azacitidine; myelodysplastic syndromes; diploidy; cells; parameters; genotyping; humans; human; male; article |
| Journal Title: | Blood |
| Volume: | 141 |
| Issue: | 11 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2023-03-16 |
| Start Page: | 1316 |
| End Page: | 1321 |
| Language: | English |
| DOI: | 10.1182/blood.2022018602 |
| PUBMED: | 36493342 |
| PROVIDER: | scopus |
| PMCID: | PMC10651766 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
