Balancing act of a leading strand DNA polymerase-specific domain and its exonuclease domain promotes genome-wide sister replication fork symmetry Journal Article

   

Authors:	Meng, X.; Claussin, C.; Regan-Mochrie, G.; Whitehouse, I.; Zhao, X.
Article Title:	Balancing act of a leading strand DNA polymerase-specific domain and its exonuclease domain promotes genome-wide sister replication fork symmetry
Abstract:	Pol2 is the leading-strand DNA polymerase in budding yeast. Here we describe an antagonism between its conserved POPS (Pol2 family-specific catalytic core peripheral subdomain) and exonuclease domain and the importance of this antagonism in genome replication. We show that multiple defects caused by POPS mutations, including impaired growth and DNA synthesis, genome instability, and reliance on other genome maintenance factors, were rescued by exonuclease inactivation. Single-molecule data revealed that the rescue stemmed from allowing sister replication forks to progress at equal rates. Our data suggest that balanced activity of Pol2's POPS and exonuclease domains is vital for genome replication and stability. © 2023 Meng et al.; Published by Cold Spring Harbor Laboratory Press.
Keywords:	genetics; mutation; dna replication; metabolism; genomic instability; exonuclease; exonucleases; dna directed dna polymerase alpha; dna polymerase ii; humans; human; pol2; exo domain; replication elongation; sister fork asymmetry
Journal Title:	Genes and Development
Volume:	37
Issue:	3-4
ISSN:	0890-9369
Publisher:	Cold Spring Harbor Laboratory Press
Date Published:	2023-02-01
Start Page:	74
End Page:	79
Language:	English
DOI:	10.1101/gad.350054.122
PUBMED:	36702483
PROVIDER:	scopus
PMCID:	PMC10069448
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85150665535&doi=10.1101%2fgad.350054.122&partnerID=40&md5=b092dc30794c04aa66c9f4920a49ba9e
Notes:	Article -- Source: Scopus

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