Harnessing split-inteins as a tool for the selective modification of surface receptors in live cells Journal Article
| Authors: | Ray, D. M.; Flood, J. R.; David, Y. |
| Article Title: | Harnessing split-inteins as a tool for the selective modification of surface receptors in live cells |
| Abstract: | Biochemical studies of integral membrane proteins are often hampered by low purification yields and technical limitations such as aggregation causing in vitro manipulations to be challenging. The ability of controlling proteins in live cells bypasses these limitations while broadening the scope of accessible questions owing to the proteins being in their native environment. Here we take advantage of the intein biorthogonality to mammalian systems, site specificity, fast kinetics, and auto-processing nature as an attractive option for modifying surface proteins. Using EGFR as a model, we demonstrate that the split-intein pair AvaN/NpuC can be used to efficiently and specifically modify target membrane proteins with a synthetic adduct for downstream live cell application. © 2022 Wiley-VCH GmbH. |
| Keywords: | animal; animals; membrane proteins; mammal; membrane protein; egfr; mammals; inteins; protein engineering; intein; protein modifications; protein splicing; split intein |
| Journal Title: | ChemBioChem |
| Volume: | 24 |
| Issue: | 3 |
| ISSN: | 1439-4227 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2023-02-01 |
| Start Page: | e202200487 |
| Language: | English |
| DOI: | 10.1002/cbic.202200487 |
| PUBMED: | 36178424 |
| PROVIDER: | scopus |
| PMCID: | PMC9977608 |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Yael David -- Export Date: 1 March 2023 -- Source: Scopus |
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