An immunocompetent rectal cancer model to study radiation therapy Journal Article
| Authors: | Kim, J. K.; Wu, C.; Del Latto, M.; Gao, Y.; Choi, S. H.; Kierstead, M.; Gabriel Sauvé, C. E.; Firat, C.; Perez, A. C.; Sillanpaa, J.; Chen, C. T.; Lawrence, K. E.; Paty, P. B.; Barriga, F. M.; Wilkinson, J. E.; Shia, J.; Sawyers, C. L.; Lowe, S. W.; García-Aguilar, J.; Romesser, P. B.; Smith, J. J. |
| Article Title: | An immunocompetent rectal cancer model to study radiation therapy |
| Abstract: | We describe a mouse model of rectal cancer (RC) involving rapid tumor organoid engraftment via orthotopic transplantation in an immunocompetent setting. This approach uses simple mechanical disruption to allow engraftment, avoiding the use of dextran sulfate sodium. The resulting RC tumors invaded from the mucosal surface and metastasized to distant organs. Histologically, the tumors closely resemble human RC and mirror remodeling of the tumor microenvironment in response to radiation. This murine RC model thus recapitulates key aspects of human RC pathogenesis and presents an accessible approach for more physiologically accurate, preclinical efficacy studies. © 2022 The Authors |
| Keywords: | radiation; rectal cancer; tumor microenvironment; tumor organoids; immunocompetent mouse model |
| Journal Title: | Cell Reports Methods |
| Volume: | 2 |
| Issue: | 12 |
| ISSN: | 2667-2375 |
| Publisher: | Cell Press |
| Date Published: | 2022-12-19 |
| Start Page: | 100353 |
| Language: | English |
| DOI: | 10.1016/j.crmeth.2022.100353 |
| PROVIDER: | scopus |
| PMCID: | PMC9795330 |
| PUBMED: | 36590695 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 3 January 2023 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work