Three-antibody classifier for muscle invasive urothelial carcinoma and its correlation with p53 expression Journal Article
| Authors: | Olkhov-Mitsel, E.; Hodgson, A.; Liu, S. K.; Vesprini, D.; Xu, B.; Downes, M. R. |
| Article Title: | Three-antibody classifier for muscle invasive urothelial carcinoma and its correlation with p53 expression |
| Abstract: | Aims To assess the utility of a three-antibody immunohistochemistry panel to classify muscle invasive bladder cancers (MIBCs) in correlation with morphological features and p53 status. Methods A retrospective review of 243 chemotherapy naïve MIBC cystectomy specimens was performed to assess morphological features. A tissue microarray was sequentially stained with CK5/6, GATA-3 and p16. Subgroups were assigned as basal-like (CK5/6+, GATA3-) and luminal (CK5/6-, GATA3+), with the latter subdivided into genomically unstable (GU, p16+) and urothelial like (Uro, p16-) subgroups. p53 staining was assessed as abnormal/wild type. Cases from the The Cancer Genome Atlas (TCGA) portal were assessed as external validation. Results We identified 78.8% luminal, 21.2% basal cases within our cohort and 63.4% luminal, 36.6% basal in the TCGA dataset. Divergent differentiation (p<0.001) was significantly associated with basal-subtype cases in both cohorts. Within the luminal subgroup (n=186), 81 cases were classified as GU and 105 as Uro. Abnormal p53 staining was noted in 48.0% of basal, 80.2% GU and 38.1% Uro cases. Further, basal-subtype tumours significantly correlated with disease-specific death compared with Uro cases in multivariate survival analysis. Conclusions This retrospective study demonstrates the potential utility of a three-antibody immunohistochemistry panel to differentiate luminal and basal MIBC. © |
| Keywords: | immunohistochemistry; cancer chemotherapy; cancer survival; controlled study; human tissue; protein expression; survival analysis; cancer surgery; retrospective studies; major clinical study; genetics; histopathology; metabolism; transcription factor gata 3; gene expression; pathology; retrospective study; wild type; protein p53; tumor marker; bladder tumor; urinary bladder neoplasms; morphology; chemistry; cystectomy; urinary bladder; tumor suppressor protein p53; surgery; tissue microarray; carcinoma, transitional cell; transitional cell carcinoma; muscle; muscles; molecular; muscle invasive bladder cancer; classifier; humans; prognosis; human; article; biomarkers, tumor |
| Journal Title: | Journal of Clinical Pathology |
| Volume: | 75 |
| Issue: | 11 |
| ISSN: | 0021-9746 |
| Publisher: | BMJ Publishing Group Ltd. |
| Date Published: | 2022-11-01 |
| Start Page: | 766 |
| End Page: | 771 |
| Language: | English |
| DOI: | 10.1136/jclinpath-2021-207573 |
| PUBMED: | 34103388 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 December 2022 -- Source: Scopus |
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