Multiplexed molecular imaging with surface enhanced resonance Raman scattering nanoprobes reveals immunotherapy response in mice via multichannel image segmentation Journal Article

   

Authors:	Andreou, C.; Plakas, K.; Berisha, N.; Gigoux, M.; Rosch, L. E.; Mirsafavi, R.; Oseledchyk, A.; Pal, S.; Zamarin, D.; Merghoub, T.; Detty, M. R.; Kircher, M. F.
Article Title:	Multiplexed molecular imaging with surface enhanced resonance Raman scattering nanoprobes reveals immunotherapy response in mice via multichannel image segmentation
Abstract:	Visualizing the presence and distribution of multiple specific molecular markers within a tumor can reveal the composition of its microenvironment, inform diagnosis, stratify patients, and guide treatment. Raman imaging with multiple molecularly-targeted surface enhanced Raman scattering (SERS) nanoprobes could help investigate emerging cancer treatments preclinically or enable personalized treatment assessment. Here, we report a comprehensive strategy for multiplexed imaging using SERS nanoprobes and machine learning (ML) to monitor the early effects of immune checkpoint blockade (ICB) in tumor-bearing mice. We used antibody-functionalized SERS nanoprobes to visualize 7 + 1 immunotherapy-related targets simultaneously. The multiplexed images were spectrally resolved and then spatially segmented into superpixels based on the unmixed signals. The superpixels were used to train ML models, leading to the successful classification of mice into treated and untreated groups, and identifying tumor regions with variable responses to treatment. This method may help predict treatment efficacy in tumors and identify areas of tumor variability and therapy resistance. © 2022 The Royal Society of Chemistry.
Keywords:	molecular imaging; molecular marker; image enhancement; tumors; diagnosis; patient treatment; mammals; image segmentation; nanoprobes; microenvironments; raman scattering; surface scattering; machine-learning; raman imaging; surface-enhanced resonance raman scattering; superpixels; early effects; enhanced raman scattering; multichannel image segmentation; super pixels; surface enhanced raman
Journal Title:	Nanoscale Horizons
Volume:	7
Issue:	12
ISSN:	2055-6756
Publisher:	Royal Society of Chemistry
Date Published:	2022-12-01
Language:	English
DOI:	10.1039/d2nh00331g
PUBMED:	36285605
PROVIDER:	scopus
PMCID:	PMC10360075
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85141689421&doi=10.1039%2fd2nh00331g&partnerID=40&md5=3633b2107153116efd4f0f71381e50e0
Notes:	Article -- Export Date: 1 December 2022 -- Source: Scopus

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