Lactobacillus supports Clostridiales to restrict gut colonization by multidrug-resistant Enterobacteriaceae Journal Article
| Authors: | Djukovic, A.; Garzón, M. J.; Canlet, C.; Cabral, V.; Lalaoui, R.; García-Garcerá, M.; Rechenberger, J.; Tremblay-Franco, M.; Peñaranda, I.; Puchades-Carrasco, L.; Pineda-Lucena, A.; González-Barberá, E. M.; Salavert, M.; López-Hontangas, J. L.; Sanz, M. Á; Sanz, J.; Kuster, B.; Rolain, J. M.; Debrauwer, L.; Xavier, K. B.; Xavier, J. B.; Ubeda, C. |
| Article Title: | Lactobacillus supports Clostridiales to restrict gut colonization by multidrug-resistant Enterobacteriaceae |
| Abstract: | Infections by multidrug-resistant Enterobacteriaceae (MRE) are life-threatening to patients. The intestinal microbiome protects against MRE colonization, but antibiotics cause collateral damage to commensals and open the way to colonization and subsequent infection. Despite the significance of this problem, the specific commensals and mechanisms that restrict MRE colonization remain largely unknown. Here, by performing a multi-omic prospective study of hospitalized patients combined with mice experiments, we find that Lactobacillus is key, though not sufficient, to restrict MRE gut colonization. Lactobacillus rhamnosus and murinus increase the levels of Clostridiales bacteria, which induces a hostile environment for MRE growth through increased butyrate levels and reduced nutrient sources. This mechanism of colonization resistance, an interaction between Lactobacillus spp. and Clostridiales involving cooperation between microbiota members, is conserved in mice and patients. These results stress the importance of exploiting microbiome interactions for developing effective probiotics that prevent infections in hospitalized patients. © 2022, The Author(s). |
| Keywords: | adult; controlled study; antibiotic therapy; gene sequence; nonhuman; comparative study; prospective study; prospective studies; mouse; animal; animals; mice; animal experiment; antiinfective agent; antibiotic resistance; anti-bacterial agents; comorbidity; probiotic agent; bacterial colonization; vancomycin; nutrient; gastrointestinal tract; nuclear magnetic resonance; colonization; butyric acid; antibiotics; metabolomics; neomycin; ampicillin; lactobacillus; probiotics; enterobacteriaceae; butyric acid derivative; butyrates; clostridiales; human; female; article; multidrug resistant enterobacteriaceae; lactobacillus rhamnosus; lactobacillus murinus |
| Journal Title: | Nature Communications |
| Volume: | 13 |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2022-09-24 |
| Start Page: | 5617 |
| Language: | English |
| DOI: | 10.1038/s41467-022-33313-w |
| PUBMED: | 36153315 |
| PROVIDER: | scopus |
| PMCID: | PMC9509339 |
| DOI/URL: | |
| Notes: | PDF misformats Joao Debivar Xavier's middle initial -- Source: Scopus |
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