PD-1 improves accurate detection of Sezary cells by flow cytometry in peripheral blood in mycosis fungoides/Sezary syndrome Journal Article


Authors: Lewis, N. E.; Gao, Q.; Petrova-Drus, K.; Pulitzer, M.; Sigler, A.; Baik, J.; Moskowitz, A. J.; Horwitz, S. M.; Dogan, A.; Roshal, M.
Article Title: PD-1 improves accurate detection of Sezary cells by flow cytometry in peripheral blood in mycosis fungoides/Sezary syndrome
Abstract: Background Accurate Sezary cell detection in peripheral blood of mycosis fungoides/Sezary syndrome (MF/SS) patients by flow cytometry can be difficult due to overlapping immunophenotypes with normal T cells using standard markers. We assessed the utility of programmed death-1 (PD-1/CD279), a transmembrane protein expressed in some hematopoietic cells, for identification and quantitation of circulating Sezary cells among established markers using flow cytometry. Methods 50 MF/SS and 20 control blood samples were immunophenotyped by flow cytometry. Principal component analysis (PCA) assessed contributions of antigens to separation of abnormal from normal T cell populations. PD-1 was assessed over time in blood and bone marrow of available MF/SS cases. Results Normal CD4+ T cells showed dim/intermediate to absent PD-1 expression. PD-1 in Sezary cells was informatively brighter (>= 1/3 log) than internal normal CD4+ T cells in 39/50 (78%) cases. By PCA, PD-1 ranked 3rd behind CD7 and CD26 in population separation as a whole; it ranked in the top 3 markers in 32/50 (64%) cases and 1st in 4/50 (8%) cases when individual abnormal populations were compared to total normal CD4+ T cells. PD-1 clearly separated Sezary from normal CD4+ T cells in 15/26 (58%, 30% of total) cases with few and subtle alterations of pan-T cell antigens/CD26 and was critical in 6 (12% of total), without which identification and quantification were significantly affected or nearly impossible. PD-1 remained informative in blood/bone marrow over time in most patients. Conclusions PD-1 significantly contributes to accurate flow cytometric Sezary cell assessment in a routine Sezary panel.
Keywords: flow cytometry; classification; lymphoma; mycosis fungoides; pd-1; t-cells; pathway; clonality; markers; sezary syndrome; biopsies; differential expression; programmed death-1
Journal Title: Cytometry Part B—Clinical Cytometry
Volume: 102
Issue: 3
ISSN: 1552-4949
Publisher: Wiley Blackwell  
Date Published: 2022-05-01
Start Page: 189
End Page: 198
Language: English
ACCESSION: WOS:000784455700001
DOI: 10.1002/cyto.b.22070
PROVIDER: wos
PMCID: PMC9162159
PUBMED: 35451196
Notes: PDF incorrectly formats Jee Yeon Baik's first name -- Source: Wos
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MSK Authors
  1. Melissa P Pulitzer
    203 Pulitzer
  2. Steven M Horwitz
    645 Horwitz
  3. Alison Moskowitz
    339 Moskowitz
  4. Ahmet Dogan
    455 Dogan
  5. Mikhail Roshal
    230 Roshal
  6. Qi   Gao
    66 Gao
  7. Natasha Elizabeth Lewis
    31 Lewis
  8. Jee Yeon Baik
    44 Baik
  9. Allison Marie Sigler
    36 Sigler