PD-1 improves accurate detection of Sezary cells by flow cytometry in peripheral blood in mycosis fungoides/Sezary syndrome Journal Article
| Authors: | Lewis, N. E.; Gao, Q.; Petrova-Drus, K.; Pulitzer, M.; Sigler, A.; Baik, J.; Moskowitz, A. J.; Horwitz, S. M.; Dogan, A.; Roshal, M. |
| Article Title: | PD-1 improves accurate detection of Sezary cells by flow cytometry in peripheral blood in mycosis fungoides/Sezary syndrome |
| Abstract: | Background Accurate Sezary cell detection in peripheral blood of mycosis fungoides/Sezary syndrome (MF/SS) patients by flow cytometry can be difficult due to overlapping immunophenotypes with normal T cells using standard markers. We assessed the utility of programmed death-1 (PD-1/CD279), a transmembrane protein expressed in some hematopoietic cells, for identification and quantitation of circulating Sezary cells among established markers using flow cytometry. Methods 50 MF/SS and 20 control blood samples were immunophenotyped by flow cytometry. Principal component analysis (PCA) assessed contributions of antigens to separation of abnormal from normal T cell populations. PD-1 was assessed over time in blood and bone marrow of available MF/SS cases. Results Normal CD4+ T cells showed dim/intermediate to absent PD-1 expression. PD-1 in Sezary cells was informatively brighter (>= 1/3 log) than internal normal CD4+ T cells in 39/50 (78%) cases. By PCA, PD-1 ranked 3rd behind CD7 and CD26 in population separation as a whole; it ranked in the top 3 markers in 32/50 (64%) cases and 1st in 4/50 (8%) cases when individual abnormal populations were compared to total normal CD4+ T cells. PD-1 clearly separated Sezary from normal CD4+ T cells in 15/26 (58%, 30% of total) cases with few and subtle alterations of pan-T cell antigens/CD26 and was critical in 6 (12% of total), without which identification and quantification were significantly affected or nearly impossible. PD-1 remained informative in blood/bone marrow over time in most patients. Conclusions PD-1 significantly contributes to accurate flow cytometric Sezary cell assessment in a routine Sezary panel. |
| Keywords: | flow cytometry; classification; lymphoma; mycosis fungoides; pd-1; t-cells; pathway; clonality; markers; sezary syndrome; biopsies; differential expression; programmed death-1 |
| Journal Title: | Cytometry Part B—Clinical Cytometry |
| Volume: | 102 |
| Issue: | 3 |
| ISSN: | 1552-4949 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2022-05-01 |
| Start Page: | 189 |
| End Page: | 198 |
| Language: | English |
| ACCESSION: | WOS:000784455700001 |
| DOI: | 10.1002/cyto.b.22070 |
| PROVIDER: | wos |
| PMCID: | PMC9162159 |
| PUBMED: | 35451196 |
| Notes: | PDF incorrectly formats Jee Yeon Baik's first name -- Source: Wos |
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