Hyperpolarized [5-(13)C,4,4-(2)H(2),5-(15)N]-L-glutamine provides a means of annotating in vivo metabolic utilization of glutamine Journal Article
| Authors: | Eskandari, R.; Kim, N.; Mamakhanyan, A.; Saoi, M.; Zhang, G.; Berisaj, M.; Granlund, K. L.; Poot, A. J.; Cross, J.; Thompson, C. B.; Keshari, K. R. |
| Article Title: | Hyperpolarized [5-(13)C,4,4-(2)H(2),5-(15)N]-L-glutamine provides a means of annotating in vivo metabolic utilization of glutamine |
| Abstract: | SignificanceGlutamine is the most abundant amino acid in human plasma, although it is challenging to determine glutamine's metabolic fate noninvasively. In this work, we utilize established chemical methods to develop a platform for imaging glutamine metabolism using hyperpolarized magnetic resonance imaging. Using this strategy, we are able to spatially measure glutaminolysis in vivo as well as develop a biomarker for the inhibition of glutaminase. Combining this biomarker with isotope tracing metabolomics connects this inhibition to reduced glutamine contribution to the tricarboxylic acid cycle. This provides an approach for future imaging of glutamine metabolism in humans. |
| Keywords: | magnetic resonance imaging; biomarkers; biological marker; metabolism; pancreatic cancer; glutamine; metabolic imaging; citric acid cycle; metabolomics; cancer metabolism; humans; human; glutaminase |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 119 |
| Issue: | 19 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2022-05-10 |
| Start Page: | e2120595119 |
| Language: | English |
| DOI: | 10.1073/pnas.2120595119 |
| PUBMED: | 35512101 |
| PROVIDER: | scopus |
| PMCID: | PMC9172133 |
| DOI/URL: | |
| Notes: | PDF misspelled the author: Marjan Berishaj’s last name -- Source: Scopus |
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