Synthesis of (124)I-labeled epichaperome probes and assessment in visualizing pathologic protein-protein interaction networks in tumor bearing mice Journal Article
| Authors: | Sharma, S.; Kalidindi, T.; Joshi, S.; Digwal, C. S.; Panchal, P.; Burnazi, E.; Lee, S. G.; Pillarsetty, N.; Chiosis, G. |
| Article Title: | Synthesis of (124)I-labeled epichaperome probes and assessment in visualizing pathologic protein-protein interaction networks in tumor bearing mice |
| Abstract: | Epichaperomes are disease-associated pathologic scaffolds composed of tightly bound chaperones and co-chaperones. They provide opportunities for precision medicine where aberrant protein-protein interaction networks, rather than a single protein, are detected and targeted. This protocol describes the synthesis and characterization of two 124I-labeled epichaperome probes, [124I]-PU-H71 and [124I]-PU-AD, both which have translated to clinical studies. It shows specific steps in the use of these reagents to image and quantify epichaperome-positivity in tumor bearing mice through positron emission tomography. For complete details on the use and execution of this protocol, please refer to Bolaender et al. (2021), Inda et al. (2020), and Pillarsetty et al. (2019). © 2022 The Author(s) |
| Keywords: | chemistry; cell culture; model organisms; cancer; molecular/chemical probes |
| Journal Title: | STAR Protocols |
| Volume: | 3 |
| Issue: | 2 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2022-06-17 |
| Start Page: | 101318 |
| Language: | English |
| DOI: | 10.1016/j.xpro.2022.101318 |
| PROVIDER: | scopus |
| PMCID: | PMC9046997 |
| PUBMED: | 35496791 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 May 2022 -- Source: Scopus |
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