LINC00355 regulates p27(KIP) expression by binding to MENIN to induce proliferation in late-stage relapse breast cancer Journal Article
| Authors: | Eteleeb, A. M.; Thunuguntla, P. K.; Gelev, K. Z.; Tang, C. Y.; Rozycki, E. B.; Miller, A.; Lei, J. T.; Jayasinghe, R. G.; Dang, H. X.; White, N. M.; Reis-Filho, J. S.; Mardis, E. R.; Ellis, M. J.; Ding, L.; Silva-Fisher, J. M.; Maher, C. A. |
| Article Title: | LINC00355 regulates p27(KIP) expression by binding to MENIN to induce proliferation in late-stage relapse breast cancer |
| Abstract: | Late-stage relapse (LSR) in patients with breast cancer (BC) occurs more than five years and up to 10 years after initial treatment and has less than 30% 5-year relative survival rate. Long non-coding RNAs (lncRNAs) play important roles in BC yet have not been studied in LSR BC. Here, we identify 1127 lncRNAs differentially expressed in LSR BC via transcriptome sequencing and analysis of 72 early-stage and 24 LSR BC patient tumors. Decreasing expression of the most up-regulated lncRNA, LINC00355, in BC and MCF7 long-term estrogen deprived cell lines decreases cellular invasion and proliferation. Subsequent mechanistic studies show that LINC00355 binds to MENIN and changes occupancy at the CDKN1B promoter to decrease p27Kip. In summary, this is a key study discovering lncRNAs in LSR BC and LINC00355 association with epigenetic regulation and proliferation in BC. © 2022, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply. |
| Keywords: | controlled study; protein expression; survival rate; gene mutation; human cell; promoter region; clinical trial; cancer patient; cancer staging; flow cytometry; cell proliferation; electron microscopy; cell viability; proteasome inhibitor; breast cancer; gene expression; small interfering rna; aromatase inhibitor; estrogen; relapse; dna methylation; rna binding protein; microarray analysis; epigenetics; chromatin immunoprecipitation; cyclin dependent kinase inhibitor 1b; western blotting; immunoprecipitation; real time polymerase chain reaction; upregulation; matrigel; hydrocortisone; transcriptome; retrovirus infection; methionine; transcriptome sequencing; dna methyltransferase 3a; calcium phosphate; streptomycin; pyruvate sodium; paraformaldehyde; lipofectamine; sanger sequencing; gene knockdown; human; female; article; long untranslated rna; ribonuclease inhibitor; mtt assay; mcf-7 cell line; pyroxylin; t-47d cell line; transwell assay; deoxycholate sodium; rna pull-down |
| Journal Title: | npj Breast Cancer |
| Volume: | 8 |
| ISSN: | 2374-4677 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2022-04-13 |
| Start Page: | 49 |
| Language: | English |
| DOI: | 10.1038/s41523-022-00412-2 |
| PROVIDER: | scopus |
| PMCID: | PMC9007952 |
| PUBMED: | 35418131 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 May 2022 -- Source: Scopus |
