Phenotypic and molecular states of IDH1 mutation-induced CD24-positive glioma stem-like cells Journal Article

   

Authors:	Haddock, S.; Alban, T. J.; Turcan, Ş; Husic, H.; Rosiek, E.; Ma, X.; Wang, Y.; Bale, T.; Desrichard, A.; Makarov, V.; Monette, S.; Wu, W.; Gardner, R.; Manova, K.; Boire, A.; Chan, T. A.
Article Title:	Phenotypic and molecular states of IDH1 mutation-induced CD24-positive glioma stem-like cells
Abstract:	Mutations in IDH1 and IDH2 drive the development of gliomas. These genetic alterations promote tumor cell renewal, disrupt differentiation states, and induce stem-like properties. Understanding how this phenotypic reprogramming occurs remains an area of high interest in glioma research. Previously, we showed that IDH mutation results in the development of a CD24-positive cell population in gliomas. Here, we demonstrate that this CD24-positive population possesses striking stem-like properties at the molecular and phenotypic levels. We found that CD24 expression is associated with stem-like features in IDH-mutant tumors, a patient-derived gliomasphere model, and a neural stem cell model of IDH1-mutant glioma. In orthotopic models, CD24-positive cells display enhanced tumor initiating potency compared to CD24-negative cells. Furthermore, CD24 knockdown results in changes in cell viability, proliferation rate, and gene expression that closely resemble a CD24-negative phenotype. Our data demonstrate that induction of a CD24-positive population is one mechanism by which IDH-mutant tumors acquire stem-like properties. These findings have significant implications for our understanding of the molecular underpinnings of IDH-mutant gliomas. © 2022
Keywords:	neural stem cells; cancer stem cells; low-grade glioma; idh1 mutation; cd24; orthotopic mouse model
Journal Title:	NeoPlasia
Volume:	28
ISSN:	1522-8002
Publisher:	Elsevier Science Inc.
Date Published:	2022-06-01
Start Page:	100790
Language:	English
DOI:	10.1016/j.neo.2022.100790
PROVIDER:	scopus
PMCID:	PMC9014446
PUBMED:	35398668
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127822951&doi=10.1016%2fj.neo.2022.100790&partnerID=40&md5=03a5e6a1284123484e2fd24e104d1248
Notes:	Article -- Export Date: 2 May 2022 -- Source: Scopus

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