SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling Journal Article

   


Authors: Gu, W.; Wang, H.; Huang, X.; Kraiczy, J.; Singh, P. N. P.; Ng, C.; Dagdeviren, S.; Houghton, S.; Pellon-Cardenas, O.; Lan, Y.; Nie, Y.; Zhang, J.; Banerjee, K. K.; Onufer, E. J.; Warner, B. W.; Spence, J.; Scherl, E.; Rafii, S.; Lee, R. T.; Verzi, M. P.; Redmond, D.; Longman, R.; Helin, K.; Shivdasani, R. A.; Zhou, Q.
Article Title: SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling
Abstract: Adult stem cells maintain regenerative tissue structure and function by producing tissue-specific progeny, but the factors that preserve their tissue identities are not well understood. The small and large intestines differ markedly in cell composition and function, reflecting their distinct stem cell populations. Here we show that SATB2, a colon-restricted chromatin factor, singularly preserves LGR5+ adult colonic stem cell and epithelial identity in mice and humans. Satb2 loss in adult mice leads to stable conversion of colonic stem cells into small intestine ileal-like stem cells and replacement of the colonic mucosa with one that resembles the ileum. Conversely, SATB2 confers colonic properties on the mouse ileum. Human colonic organoids also adopt ileal characteristics upon SATB2 loss. SATB2 regulates colonic identity in part by modulating enhancer binding of the intestinal transcription factors CDX2 and HNF4A. Our study uncovers a conserved core regulator of colonic stem cells able to mediate cross-tissue plasticity in mature intestines. © 2021 Elsevier Inc.
Keywords: satb2, intestine regeneration, colonic mucosa, stem cell conversion, enhancer remodelingintestine
Journal Title: Cell Stem Cell
Volume: 29
Issue: 1
ISSN: 1934-5909
Publisher: Cell Press  
Date Published: 2022-01-06
Start Page: 101
End Page: 115.e10
Language: English
DOI: 10.1016/j.stem.2021.09.004
PUBMED: 34582804
PROVIDER: scopus
PMCID: PMC8741647
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121988081&doi=10.1016%2fj.stem.2021.09.004&partnerID=40&md5=935ea70ad7df1da6023895c177032eb2
Notes: Article -- Export Date: 1 February 2022 -- Source: Scopus
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MSK Authors
  1. Kristian Helin
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  2. Hua Wang
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