Delivery of membrane impermeable molecules to primary mouse T lymphocytes Review
| Authors: | Xu, K.; Li, M. O. |
| Review Title: | Delivery of membrane impermeable molecules to primary mouse T lymphocytes |
| Abstract: | The pore-forming toxin streptolysin-O (SLO) enables intracellular delivery of molecules up to 100 kDa and has been used for short-term delivery of membrane-impermeable substances to assess their effects on cellular activities. A limitation of this technique is the loss of intracellular components and the potential unpredicted alterations of cellular metabolism and signaling. This protocol, optimized for primary mouse T lymphocytes, describes steps for SLO-mediated cell membrane permeabilization and substance supplementation, followed by immunoblotting and immunofluorescent microscopy for assessing cellular effects. For complete details on the use and execution of this protocol, please refer to Xu et al. (2021a and 2021b). © 2021 The Author(s) |
| Keywords: | signal transduction; microscopy; metabolism; immunology; cell isolation; cell biology; flow cytometry/mass cytometry |
| Journal Title: | STAR Protocols |
| Volume: | 2 |
| Issue: | 3 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2021-09-17 |
| Start Page: | 100757 |
| Language: | English |
| DOI: | 10.1016/j.xpro.2021.100757 |
| PROVIDER: | scopus |
| PMCID: | PMC8377589 |
| PUBMED: | 34458872 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 February 2022 -- Source: Scopus |
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