Synapse - PARP1: A potential molecular marker to identify cancer during colposcopy procedures

PARP1: A potential molecular marker to identify cancer during colposcopy procedures Journal Article

Authors:	de Souza França, P. D.; Guru, N.; Kostolansky, A. R.; Mauguen, A.; Pirovano, G.; Kossatz, S.; Roberts, S.; Abrahão, M.; Patel, S. G.; Park, K. J.; Reiner, T.; Jewell, E.
Article Title:	PARP1: A potential molecular marker to identify cancer during colposcopy procedures
Abstract:	Despite efforts in prevention, cervical cancer still presents with a high worldwide incidence and remains a great problem in public health, especially in low-income countries. Screening programs, such as colposcopy with Papanicolaou testing, have greatly improved mortality rates. However, the agents currently used to delineate those lesions (topical application of acetic acid or Lugol iodine) lack specificity and sometimes can lead to unnecessary biopsies or even cervical excisions. A tool to enable in vivo histology to quickly and quantitatively distinguish between tumor, dysplastic tissue, and healthy tissue would be of great clinical interest. Methods: Here, we describe the use of PARPi-FL, a fluorescent inhibitor of poly[adenosine diphosphateribose]polymerase 1 (PARP1), which is a nuclear enzyme that is over expressed in cancer when compared with the normal surrounding tissues. We exploit its use as an optical imaging agent to specifically target PARP1 expression, which was demonstrated to be higher in cervical cancer than the normal surrounding tissue. Results: After topical application of PARPi-FL on freshly excised cone biopsy samples, the nuclei of tumor cells emitted a specific fluorescent signal that could be visualized using a handheld fluorescence confocal microscope. Conclusion: This approach has the potential to improve in vivo identification of tumor cells during colposcopy examination, allowing a rapid, noninvasive, and accurate histopathologic assessment.
Keywords:	income; cervical cancer; parp1; parpi-fl; fluorescence-guided surgery
Journal Title:	Journal of Nuclear Medicine
Volume:	62
Issue:	7
ISSN:	0161-5505
Publisher:	Society of Nuclear Medicine
Date Published:	2021-07-01
Start Page:	941
End Page:	948
Language:	English
ACCESSION:	WOS:000709477900014
DOI:	10.2967/jnumed.120.253575
PROVIDER:	wos
PUBMED:	33188153
PMCID:	PMC8882878
Notes:	Article -- Source: Wos