In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site Journal Article

   


Authors: Wobbe, C. R.; Dean, F.; Weissbach, L.; Hurwitz, J.
Article Title: In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site
Abstract: Extracts (0.2 M NaCl) of HeLa cells support replication of DNA containing simian virus 40 (SV40) origin in the presence of SV40 large tumor (T) antigen. The reaction leads to the accumulation of high molecular weight products that represent DNA containing one parental strand and one progeny strand as well as duplex molecules that contain both strands derived from the input deoxynucleoside triphosphates. The replication reaction is inhibited by aphidicolin and by camptothecin, two inhibitors known to inhibit eukaryotic DNA replication in vivo.
Keywords: nonhuman; dna replication; in vitro study; cell culture; eukaryota; simian virus 40; cell extract; simiae; simian virus; priority journal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 82
Issue: 17
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1985-09-01
Start Page: 5710
End Page: 5714
Language: English
DOI: 10.1073/pnas.82.17.5710
PROVIDER: scopus
PMCID: PMC390621
PUBMED: 2994044
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0010227867&doi=10.1073%2fpnas.82.17.5710&partnerID=40&md5=c05f21c5e125ebed22e175a263877935
Notes: Article -- Export Date: 26 October 2021 -- Source: Scopus
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MSK Authors
  1. Jerard Hurwitz
    206 Hurwitz
  2. Frank B. Dean
    23 Dean
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