Activation of S6 kinase activity in 3T3-L1 cells by insulin and phorbol ester Journal Article
| Authors: | Tabarini, D.; Heinrich, J.; Rosen, O. M. |
| Article Title: | Activation of S6 kinase activity in 3T3-L1 cells by insulin and phorbol ester |
| Abstract: | Treatment of 3T3-L1 cells with 0.1-1.0 nM insulin results in rapid (5-15 min) activation of a soluble protein kinase that phosphorylates serine residues in ribosomal protein S6. The insulin-stimulated kinase activity is detectable in confluent, nongrowing preadipocytes and adipocytes. In the presence of 2 μg of cycloheximide per ml, preconfluent 3T3-L1 cells also respond to insulin by acquiring an S6 kinase activity whose properties are the same as those of the enzyme activity elicited by insulin alone in growth-inhibited cells. The principal insulin-stimulated S6 kinase has a M(r) of ~50,000-60,000; there is a variable amount of activity that sediments with a M(r) of about 80,000. The soluble enzyme exhibits optimal activity between pH 8 and pH 9, requires Mg2+ (10-20 mM), and is inhibited by Ca2+ (0.5 mM), Mn2+ (0.05 mM), and NaF (30 mM). GTP cannot substitute for ATP in the phosphotransferase reaction; cAMP, cGMP, phosphatidylserine plus diolein, the cAMP-dependent protein kinase inhibitor, and heparin (0.7 μg/ml) are without effect. Although treatment of 3T3-L1 cells with insulin does not influence the activity or the subcellular distribution of the phospholipid and Ca2+-dependent protein kinase C, exposure to the phorbol tumor promoter phorbol 12-myristate 13-acetate (PMA) results in translocation of protein kinase C to the membrane and activation of a soluble phospholipid and Ca2+-independent S6 protein kinase that has the same magnitude of activity and sedimentation behavior as the insulin-induced activity. Trypsin treatment of either 3T3-L1 cytosolic extracts or partially purified 3T3-L1 protein kinase C generates a small amount of S6 kinase activity of M(r) 50,000. This activity, resolved by sucrose gradient centrifugation, is less active than that elicited by either insulin or PMA and, unlike the activities generated by insulin and PMA, is associated with histone kinase activity. The data suggest that the S6 kinase elicited by either insulin or PMA is neither protein kinase C, its phospholipid, and Ca2+-independent proteolytic derivative nor the result of proteolytic activation of an inactive proenzyme that can be reproduced by trypsin treatment of cell extracts in vitro. |
| Keywords: | protein phosphorylation; nonhuman; mouse; enzyme activation; in vitro study; cell culture; insulin; protein kinase; ribosome protein; phorbol ester; phorbol 13 acetate 12 myristate; subcutaneous tissue; cycloheximide; priority journal |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 82 |
| Issue: | 13 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1985-07-01 |
| Start Page: | 4369 |
| End Page: | 4373 |
| Language: | English |
| DOI: | 10.1073/pnas.82.13.4369 |
| PROVIDER: | scopus |
| PMCID: | PMC390415 |
| PUBMED: | 3892533 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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