Site-specifically modified oligodeoxyribonucleotides as templates for Escherichia coli DNA polymerase I Journal Article
| Authors: | O'Connor, D.; Stöhrer, G. |
| Article Title: | Site-specifically modified oligodeoxyribonucleotides as templates for Escherichia coli DNA polymerase I |
| Abstract: | Oligodeoxyribonucleotides with site-specific modifications have been used as substrates for Escherichia coli DNA polymerase I holoenzyme and Klenow fragment. Modifications included the bulky guanine-8-aminofluorene adduct and a guanine oxidation product resembling the product of photosensitized DNA oxidation. By a combination of primers and 'nick-mers', conditions of single-strand-directed DNA synthesis and nick-translation could be created. Our results show that the polymerase can bypass both types of lesions. Bypass occurs on a single-stranded template but is facilitated on a nicked, double-stranded template. Only purines, with guanine more favored than adenine, are incorporated across both lesions. Hesitation during bypass could not be detected. The results indicate that site-specifically modified oligonucleotides can be sensitive probes for the action of polymerases on damaged templates. They also suggest a function for polymerase I, in its nick-translation capacity, during DNA repair and mutagenesis. |
| Keywords: | nonhuman; dna polymerase; heredity; escherichia coli; mutant; priority journal; oligodeoxyribonucleotide |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 82 |
| Issue: | 8 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1985-04-01 |
| Start Page: | 2325 |
| End Page: | 2329 |
| Language: | English |
| DOI: | 10.1073/pnas.82.8.2325 |
| PROVIDER: | scopus |
| PMCID: | PMC397550 |
| PUBMED: | 3887400 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work