Evidence for the cerebral uptake in vivo from two pools of glucose and the role of glucose-6-phosphatase in removing excess substrate from brain Journal Article
| Authors: | Sacks, W.; Cowburn, D.; Bigler, R. E.; Sacks, S.; Fleischer, A. |
| Article Title: | Evidence for the cerebral uptake in vivo from two pools of glucose and the role of glucose-6-phosphatase in removing excess substrate from brain |
| Abstract: | We propose the following scheme for cerebral uptake and overall metabolism of glucose in vivo: that brain selects from two pools of glucose anomers in arterial blood, that it takes up excess glucose, that glucose enters the brain tissue as glucose-6-phosphate through the actions of mutarotase and hexokinase, that some glucose-6-phosphate becomes metabolized to CO2 and some becomes incorporated into brain carbon pools, and that excess glucose-6-phosphate leaves brain through glucose-6-phosphatase and mutarotase activities. This results from our observations in arterio-venous studies for the determination of cerebral metabolism in humans in vivo that the cerebral uptake of [14C]glucose often appeared to differ from that of unlabeled glucose. With rapidly falling arterial radioactivity, unlabeled glucose uptake was more than [14C]glucose. With rising arterial radioactivity, [14C]glucose extraction extraction exceeded unlabeled glucose. Studies with [14C]glucose-6-phosphate suggested that glucose-6-phosphatase in brain removes excess substrate by dephosphorylation. However, when arterial [14C]glucose increased slowly, [14C]glucose uptake varied considerably and the data resembled human cerebral metabolism of glucose anomers. An experiment employing [13C]glucose and NMR provided further support for our proposed scheme. © 1985 Plenum Publishing Corporation. |
| Keywords: | unclassified drug; nonhuman; animal cell; animals; phosphorylation; central nervous system; animalia; brain; rat; magnetic resonance spectroscopy; glucose; rats; radioisotope; spectrum analysis; hexokinase; glucose 6 phosphatase; glucose-6-phosphatase; humans; priority journal; article; glucose c 14; carbohydrate epimerases |
| Journal Title: | Neurochemical Research |
| Volume: | 10 |
| Issue: | 2 |
| ISSN: | 0364-3190 |
| Publisher: | Springer |
| Date Published: | 1985-02-01 |
| Start Page: | 201 |
| End Page: | 227 |
| Language: | English |
| DOI: | 10.1007/bf00964568 |
| PROVIDER: | scopus |
| PUBMED: | 2986020 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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