Acute effects of dietary cholic acid and methylazoxymethanol acetate on colon epithelial cell proliferation; Metabolism of bile salts and neutral sterols in conventional and germfree SD rats Journal Article
| Authors: | Weidema, W. F.; Deschner, E. E.; Cohen, B. I.; DeCosse, J. J. |
| Article Title: | Acute effects of dietary cholic acid and methylazoxymethanol acetate on colon epithelial cell proliferation; Metabolism of bile salts and neutral sterols in conventional and germfree SD rats |
| Abstract: | The acute effects of cholic acid ingestion on methylazoxymethanol acetate [(MAM) CAS: 592-62-1]-treated conventional and germfree rats were investigated. Male SD rats were divided into 4 treatment groups. The first group received control chow; the second group, control chow plus 0.5% cholic acid; the third group, control chow plus MAM; and the fourth group, control chow plus 0.5% cholic acid plus MAM. Fecal bile acids, cholesterol, cholesterol degradation products, and neutral sterols, as well as labeling indices and numbers of epithelial cells per crypt column, were measured after 6 weeks of treatment. The administration of MAM to germfree groups diminished both fecal bulk and the amount of fecal water. MAM did not affect the fecal bile acid composition. Analysis of the fecal bile acids in conventional rats fed cholic acid demonstrated that half of the bile acids were in a form of deoxycholic acid. In the germfree groups fed cholic acid, 90% of the bile acids appeared unaltered in the feces. Neither in the germfree nor in the conventional groups was an effect seen of MAM on the output of fecal neutral sterols. The addition of cholic acid to the food decreased the output of neutral sterols both in the conventional (P < 0.001) and in the germfree (P < .02) animals. The germfree animals showed a reduced amount of neutral steroid excretion (P < .01) when compared to the findings for the conventional groups. MAM had no influence on the fecal cholesterol or coprostanol output. The consumption of 0.5% cholic acid decreased the total output of cholesterol (P < .05). The excretion of coprostanol was significantly diminished in the conventional rats fed cholic acid (P < .001). No difference in labeling indices was observed between conventional and germfree rats, whether treated with cholic acid, MAM, or cholic acid plus MAM. However, all germfree groups showed less epithelial cells per crypt column (P < .001) than did conventional animals. |
| Keywords: | histopathology; nonhuman; cell proliferation; animal cell; animal; cell division; animal experiment; colonic neoplasms; histology; diet; rat; epithelium cell; rats; epithelium; feces; colon; therapy; autoradiography; carcinogen; germ-free life; large intestine; azo compounds; rats, inbred strains; male; priority journal; bile salt; cholic acids; bile acids and salts; cholic acid; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; sterol; methylazoxymethanol acetate; sterols |
| Journal Title: | JNCI: Journal of the National Cancer Institute |
| Volume: | 74 |
| Issue: | 3 |
| ISSN: | 0027-8874 |
| Publisher: | Oxford University Press |
| Date Published: | 1985-03-01 |
| Start Page: | 665 |
| End Page: | 670 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 3856068 |
| DOI: | 10.1093/jnci/74.3.665 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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