cAMP is an essential signal in the induction of antibody production by B cells but inhibits helper function of T cells Journal Article
| Authors: | Gilbert, K. M.; Hoffmann, M. K. |
| Article Title: | cAMP is an essential signal in the induction of antibody production by B cells but inhibits helper function of T cells |
| Abstract: | Dibutyryl cAMP and IL 1 were found to stimulate antigen-specific and polyclonal antibody production when added together to cultures of highly purified B cells. We propose that IL 1 and an elevation in cytoplasmic cAMP represent minimal signal requirements for B cell activation. In contrast to its effect on B cells, dibutyryl cAMP inhibited helper T cell activity. Cyclic AMP suppressed the production of IL 2 and T cell replacing factor (TRF) by T cells and thus abrogated the ability of helper T cells to enhance SRBC-specific antibody production by B cells. Cyclic AMP did not inhibit the generation by T cells of B cell growth factor (BCGF). BCGF, not normally detected in Con A supernatant, was found in the culture supernatant of spleen cells that were stimulated with Con A in the presence of cAMP. Our findings indicate that cAMP blocks the production of an inhibitor of BCGF activity. cAMP had no effect on the production by macrophages of IL 1. |
| Keywords: | nonhuman; t lymphocyte; t-lymphocytes; animal cell; mouse; animal; mice; spleen; interleukin 5; in vitro study; b lymphocyte; b-lymphocytes; lymphatic system; mice, nude; cyclic amp; t-lymphocytes, helper-inducer; antibody formation; antibody production; helper cell; interleukin-2; interleukin-1; concanavalin a; priority journal; lymphokines; support, u.s. gov't, p.h.s.; lymphocyte cooperation |
| Journal Title: | Journal of Immunology |
| Volume: | 135 |
| Issue: | 3 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1985-09-01 |
| Start Page: | 2084 |
| End Page: | 2089 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 2991378 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
