| Abstract: |
Several C18 HPLC columns, varying in column length as well as packing material diameter, were used to separate mixtures of biochemical compounds in standards and in various physiological fluids. The column performances were compared both qualitatively and quantitatively. Factors investigated included efficiency, resolution, selectivity, column effect on peak height, temperature effects, cost per analysis, and analysis times. Column lifetime was also examined. The results were used to evaluate the suitability of the various columns for use in the chromatographic analysis of physiological samples. © 1985 by Preston Technical Abstracts Company. |
| Keywords: |
blood chemical analysis; time; time factors; instrumentation; blood analysis; urine; temperature; body fluids; high performance liquid chromatography; chromatography, high pressure liquid; evaluation studies; cost; body fluid; costs and cost analysis; indicators and reagents; chromatographic analysis; dyes, reagents, indicators, markers and buffers; spectrophotometry, ultraviolet; human; article; ultraviolet spectrophotometry; support, u.s. gov't, p.h.s.; biochemistry - equipment; chemical analysis - equipment; column lifetime; packing material diameter
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