Observations on the suramin-mediated inhibition of cellular and viral DNA polymerases Journal Article
| Authors: | Basu, A.; Modak, M. J. |
| Article Title: | Observations on the suramin-mediated inhibition of cellular and viral DNA polymerases |
| Abstract: | We have examined the sensitivity of various cellular and viral DNA polymerases to Suramin, an antitrypanosomal drug, which has been reported to exhibit antireverse transcriptase activity. We find that Suramin is a nonspecific inhibitor of all the viral and cellular DNA polymerases, including terminal deoxynucleotidyl transferase, and that the inhibition is most readily reversed by the addition of serum albumin. The drug appears to bind to all the enzyme proteins with no apparent selectivity. Binding of Suramin to enzyme has been found to result in the loss of both substrate and template-primer binding abilities of various enzymes, confirming the nonspecific nature of protein-Suramin interaction. © 1985. |
| Keywords: | dose response; drug efficacy; dna polymerase; drug inhibition; enzyme inhibition; drug mechanism; escherichia coli; drug response; antivirus agent; acquired immune deficiency syndrome; dna nucleotidylexotransferase; drug binding; protozoon; suramin; retrovirus; in vitro; dna-directed dna polymerase; dna polymerase i; viruses; reverse transcriptase inhibitors; rna directed dna polymerase; human; priority journal; support, non-u.s. gov't; deoxyribonucleotides |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 128 |
| Issue: | 3 |
| ISSN: | 0006-291X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1985-05-16 |
| Start Page: | 1395 |
| End Page: | 1402 |
| Language: | English |
| DOI: | 10.1016/0006-291x(85)91095-2 |
| PUBMED: | 2408617 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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