Insights into homology search from cryo-EM structures of RecA-DNA recombination intermediates Review

   

Authors:	Yang, H.; Pavletich, N. P.
Review Title:	Insights into homology search from cryo-EM structures of RecA-DNA recombination intermediates
Abstract:	The fundamental reaction in homologous recombination is the exchange of strands between two homologous DNA molecules. This reaction is carried out by the RecA family of ATPases that polymerize on ssDNA to form a presynaptic filament. This filament then binds to dsDNA to form a synaptic filament, a key intermediate that mediates the search for homology and subsequent strand exchange to produce a new heteroduplex. A recent cryo-EM analysis of synaptic filaments has now shed light on this process. The dsDNA strands are separated on binding to the filament. One strand is sequestrated while the other is freed to sample pairing with the ssDNA. Homology, through heteroduplex formation, promotes dsDNA opening. Lack of homology suppresses it, keeping local synapses short so that multiple synapses can form and increasing the probability of encountering homology. © 2021 Elsevier Ltd
Journal Title:	Current Opinion in Genetics and Development
Volume:	71
ISSN:	0959-437X
Publisher:	Elsevier Inc.
Date Published:	2021-12-01
Start Page:	188
End Page:	194
Language:	English
DOI:	10.1016/j.gde.2021.09.002
PROVIDER:	scopus
PUBMED:	34592688
PMCID:	PMC8671187
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116006886&doi=10.1016%2fj.gde.2021.09.002&partnerID=40&md5=da088844b9e96ddf85164f78ab4fe531
Notes:	Review -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/188753