Unraveling Ewing sarcoma tumorigenesis originating from patient-derived mesenchymal stem cells Journal Article
| Authors: | Sole, A.; Grossetête, S.; Heintzé, M.; Babin, L.; Zaïdi, S.; Revy, P.; Renouf, B.; De Cian, A.; Giovannangeli, C.; Pierre-Eugène, C.; Janoueix-Lerosey, I.; Couronné, L.; Kaltenbach, S.; Tomishima, M.; Jasin, M.; Grünewald, T. G. P.; Delattre, O.; Surdez, D.; Brunet, E. |
| Article Title: | Unraveling Ewing sarcoma tumorigenesis originating from patient-derived mesenchymal stem cells |
| Abstract: | Ewing sarcoma is characterized by pathognomonic translocations, most frequently fusing EWSR1 with FLI1. An estimated 30% of Ewing sarcoma tumors also display genetic alterations in STAG2, TP53, or CDKN2A (SPC). Numerous attempts to develop relevant Ewing sarcoma models from primary human cells have been unsuccessful in faithfully recapitulating the phenotypic, transcriptomic, and epigenetic features of Ewing sarcoma. In this study, by engineering the t(11;22)(q24;q12) translocation together with a combination of SPC mutations, we generated a wide collection of immortalized cells (EWIma cells) tolerating EWSR1-FLI1 expression from primary mesenchymal stem cells (MSC) derived from a patient with Ewing sarcoma. Within this model, SPC alterations strongly favored Ewing sarcoma oncogenicity. Xenograft experiments with independent EWIma cells induced tumors and metastases in mice, which displayed bona fide features of Ewing sarcoma. EWIma cells presented balanced but also more complex translocation profiles mimicking chromoplexy, which is frequently observed in Ewing sarcoma and other cancers. Collectively, these results demonstrate that bone marrow–derived MSCs are a source of origin for Ewing sarcoma and also provide original experimental models to investigate Ewing sarcomagenesis. © 2021 American Association for Cancer Research |
| Journal Title: | Cancer Research |
| Volume: | 81 |
| Issue: | 19 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2021-10-01 |
| Start Page: | 4994 |
| End Page: | 5006 |
| Language: | English |
| DOI: | 10.1158/0008-5472.Can-20-3837 |
| PUBMED: | 34341072 |
| PROVIDER: | scopus |
| PMCID: | PMC8487988 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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