RNA splicing products formed with isolated fractions from HeLa cells are associated with fast-sedimenting complexes Journal Article

   


Authors: Perkins, K. K.; Furneaux, H. M.; Hurwitz, J.
Article Title: RNA splicing products formed with isolated fractions from HeLa cells are associated with fast-sedimenting complexes
Abstract: Three fractions (designated Ia, Ib, and II) have been isolated from HeLa cell nuclear extracts that are required for splicing of adenovirus and human β-globin RNA transcripts in vitro. The incubation of two of the fractions (Ib and II) in the presence of ATP resulted in cleavage of precursor mRNA at the 5' splice site and formation of the intron-exon lariat. Addition of fraction Ia to the combination of Ib and II resulted in the formation of spliced RNA and the intron lariat. When fraction II was incubated with precursor RNA in the presence of ATP and the resulting products were sedimented through sucrose gradients, a 30S complex was detected that contained precursor RNA. The combination of fractions Ib and II resulted in the production of a 55S complex that contained the 5' exon as a prominent RNA species. The combination of fractions I (containing Ia and Ib) and II resulted in the formation of the 55S complex and material sedimenting between 40 S and 20 S, in which the predominant RNA species was spliced RNA.
Keywords: human cell; heredity; hela cell; plasmid; cell nucleus; rna processing; adenoviridae; beta globin; adenovirus; human; priority journal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 83
Issue: 4
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1986-02-01
Start Page: 887
End Page: 891
Language: English
DOI: 10.1073/pnas.83.4.887
PUBMED: 3456573
PROVIDER: scopus
PMCID: PMC322975
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022505071&doi=10.1073%2fpnas.83.4.887&partnerID=40&md5=e2b74f1410148003f9451f7483d176b1
Notes: Article -- Export Date: 18 August 2021 -- Source: Scopus
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MSK Authors
  1. Henry M Furneaux
    57 Furneaux
  2. Jerard Hurwitz
    206 Hurwitz
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