Elevated levels of p53 antigen in cultured skin fibroblasts from patients with hereditary adenocarcinoma of the colon and rectum and its relevance to oncogenic mechanisms Journal Article
| Authors: | Kopelovich, L.; DeLeo, A. B. |
| Article Title: | Elevated levels of p53 antigen in cultured skin fibroblasts from patients with hereditary adenocarcinoma of the colon and rectum and its relevance to oncogenic mechanisms |
| Abstract: | Skin fibroblasts (SFs) from patients with adenomatosis of the colon and rectum (ACR) were shown, in general, to express elevated amounts of the p53 antigen as determined by immunoprecipitation with the monoclonal antibody PAb421. Infection with simian virus 40 (SV40) induced expression of the p53 antigen in SFs from normal individuals and further amplified its expression in ACR cells. The half-lives of the p53 antigen in mockinfected ACR cells and in SV40-transformed normal or ACR cells were about 2 and 15 hours, respectively. No immediate differences were detected in the coding segment of the human p53 gene between normal and ACR cells by either Southern or Northern blot analysis. These results suggest that over-expression of the p53 antigen is due, in part, to the increased stability of the p53 antigen in ACR cells. Over-expression represents an early event that is possibly associated with initiation and promotion of inherited colon cancer. © 1986 Oxford University Press. |
| Keywords: | human cell; adenocarcinoma; antigen expression; cells, cultured; heredity; colonic neoplasms; in vitro study; carcinogenesis; skin; antigens, neoplasm; cell culture; colon cancer; simian virus 40; fibroblasts; phosphoproteins; genetic disorder; rectal neoplasms; rectum cancer; cell transformation, viral; skin fibroblast; large intestine; human; priority journal; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s. |
| Journal Title: | JNCI: Journal of the National Cancer Institute |
| Volume: | 77 |
| Issue: | 6 |
| ISSN: | 0027-8874 |
| Publisher: | Oxford University Press |
| Date Published: | 1986-12-01 |
| Start Page: | 1241 |
| End Page: | 1246 |
| Language: | English |
| DOI: | 10.1093/jnci/77.6.1241 |
| PUBMED: | 3467115 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work