Mutagenesis originating in site-specific DNA damage Journal Article
| Authors: | Mitchell, N.; Stöhrer, G. |
| Article Title: | Mutagenesis originating in site-specific DNA damage |
| Abstract: | Covalent monoadducts are the major types of gene damage after exposure to chemical mutagens and carcinogens. These and other damage structures together give rise to the spectrum of mutations that includes base-pair substitutions, insertions and deletions. In this study we introduced the bulky adduct guanine-8-aminofluorene into defined sites on one or both strands of the lactose operator. After insertion into plasmid pBR322 and replication in Escherichia coli COEC40, operator mutants were recognized on 5-bromo,4-chloro, 3-indolyl-β-d-galactoside plates and by hydridization probing. Out of ten randomly selected mutants, nine were single-base deletions and one was a two-base deletion. All mutations were at the site of modification or immediately adjacent to that site. If modifications were placed into both plasmid strands, preventing excision repair, operator mutants comprised close to 100% of operator-containing plasmids. © 1986. |
| Keywords: | mutation; nonhuman; dna damage; heredity; genetic engineering; escherichia coli; base sequence; plasmids; dna, bacterial; operator regions (genetics); chemical mutagenesis; priority journal; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; 8 (2 fluorenylamino)deoxyguanosine; base excision |
| Journal Title: | Journal of Molecular Biology |
| Volume: | 191 |
| Issue: | 2 |
| ISSN: | 0022-2836 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 1986-09-20 |
| Start Page: | 177 |
| End Page: | 180 |
| Language: | English |
| DOI: | 10.1016/0022-2836(86)90254-8 |
| PUBMED: | 3543376 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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