Calcium mobilization and catecholamine secretion in adrenal chromaffin cells: A Quin-2 fluorescence study Journal Article
| Authors: | Kao, L. S.; Schneider, A. S. |
| Article Title: | Calcium mobilization and catecholamine secretion in adrenal chromaffin cells: A Quin-2 fluorescence study |
| Abstract: | To better understand the relation between cell calcium and exocytotic secretion, a quantitative dependence of adrenal catecholamine secretion on cytosolic free calcium has been determined for isolated, intact, bovine chromaffin cells, using the fluorescent probe Quin-2. The cells required a threshold of 250-300 nM cytosolic calcium to be reached before detectable secretion occurred and half-maximal secretion occurred near 2 μM cysotolic calcium. Nicotinic receptors mediated an increase of cytosolic calcium from resting levels near 100 nM to levels in the 1-10 μM range within seconds followed by a decay back to resting levels over several minutes. Muscarinic receptors mediated a smaller rise in cytosolic free calcium from 100 to about 200 nM, within seconds. The nicotinic response required extracellular calcium, while the muscarinic calcium, suggesting the latter mobilizes intracellular calcium. The acetylcholine-evoked rise in cytosolic calcium decayed by at least two kinetically distinct processes with half-time constants: t1 = 0.6 min and t2 = 3.2 min. Extracellular Na+ deprivation caused a more prolonged elevation of the acetylcholine-evoked calcium transient, suggesting a possible role of Na+/Ca2+ exchange and/or other Na+-dependent processes in lowering cytosolic calcium following stimulation. The possible perturbing effects of Quin-2 on resting and stimulated cytosolic calcium levels and on secretion were examined and a novel use of Quin-2 to measure membrane calcium flux was demonstrated. |
| Keywords: | nonhuman; animal cell; animal; fluorescence; fluorescent dyes; spectrometry, fluorescence; calcium; dose-response relationship, drug; kinetics; cattle; nicotine; sodium; catecholamines; cytosol; exocytosis; endocrine system; catecholamine release; calcium mobilization; aminoquinolines; adrenal glands; receptors, nicotinic; priority journal; chromaffin cell; chromaffin system; acetylcholine; support, u.s. gov't, p.h.s.; receptors, muscarinic; muscarine; quin 2; methacholine chloride; methacholine compounds |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 261 |
| Issue: | 11 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1986-04-15 |
| Start Page: | 4881 |
| End Page: | 4888 |
| Language: | English |
| PUBMED: | 3514606 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S0021-9258(19)89187-1 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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