In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation Journal Article

  


Authors: Pia Pistillo, M.; Hämmerling, U.; Dupont, B.; Battista Ferrara, G.
Article Title: In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation
Abstract: This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent. © 1986.
Keywords: cell line; in vitro study; b lymphocyte; b-lymphocytes; lymphocyte activation; lymphocytes; hla antigen; hla antigens; epstein barr virus; antibody formation; antibody production; cell transformation, viral; herpesvirus 4, human; immunization, secondary; epitopes; hla antibody; human; priority journal; virus cell transformation; isoantibodies; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; blood and hemopoietic system
Journal Title: Human Immunology
Volume: 15
Issue: 1
ISSN: 0198-8859
Publisher: Elsevier Inc.  
Date Published: 1986-01-01
Start Page: 109
End Page: 117
Language: English
DOI: 10.1016/0198-8859(86)90321-6
PUBMED: 2419282
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022629818&doi=10.1016%2f0198-8859%2886%2990321-6&partnerID=40&md5=26001b07cfb1d7c44c7f66d4455eaf1a
Notes: Article -- Export Date: 18 August 2021 -- Source: Scopus
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MSK Authors
  1. Ulrich Hammerling
    79 Hammerling
  2. Bo Dupont
    264 Dupont
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