Morphological, biological, biochemical, and karyotypic characteristics of human pancreatic ductal adenocarcinoma Capan-2 in tissue culture and the nude mouse Journal Article
| Authors: | Kyriazis, A. P.; Kyriazis, A. A.; Sternberg, C. N.; Sloane, N. H.; Loveless, J. D. |
| Article Title: | Morphological, biological, biochemical, and karyotypic characteristics of human pancreatic ductal adenocarcinoma Capan-2 in tissue culture and the nude mouse |
| Abstract: | Human pancreatic ductal adenocarcinoma Capan-2, derived from a 56-yr-old male Caucasian, has been studied in both tissue culture and the nude mouse. In tissue culture, tumor cells showed epithelial-like features, whereas in the nude mouse, the tumor grew as a well-differentiated adenocarcinoma, resembling histopathologically the original neoplasm. Ultrastructurally, the neoplastic cells showed characteristics of ductal epithelium. The allozyme phenotypic profile of Tumor Capan-2 was determined in eight genetically determined loci, and chromosome studies showed a hypotetraploid pattern with a number of morphological and numerical changes. Carcinoembryonic antigen was produced in trace amounts, and lactate dehydrogenase was represented only by Isoenzyme 5, regardless of environmental conditions. The characteristics of Capan-2 tumor make it a valuable addition to the small number of available pancreatic tumor lines in studies aiming at clarifying certain aspects of the biology of this type of malignancy. © 1986, American Association for Cancer Research. All rights reserved. |
| Keywords: | human cell; pancreatic neoplasms; pancreas; cytology; electron microscopy; animals; mice; microscopy, electron; cell division; carcinoembryonic antigen; cell line; in vitro study; nude mouse; mice, nude; tumor cell line; pancreas adenocarcinoma; lactate dehydrogenase; neoplasm transplantation; epithelium; karyotype; karyotyping; l-lactate dehydrogenase; ultrastructure; carcinoma, intraductal, noninfiltrating; enzymes; isoenzymes; characterization; tissue culture; humans; human; priority journal |
| Journal Title: | Cancer Research |
| Volume: | 46 |
| Issue: | 11 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1986-11-01 |
| Start Page: | 5810 |
| End Page: | 5815 |
| Language: | English |
| PUBMED: | 3019537 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
