Expression of epidermal growth factor receptor in human cultured cells and tissues: Relationship to cell lineage and stage of differentiation Journal Article

  

Authors:	Real, F. X.; Rettig, W. J.; Garin Chesa, P.; Melamed, M. R.; Old, L. J.; Mendelsohn, J.
Article Title:	Expression of epidermal growth factor receptor in human cultured cells and tissues: Relationship to cell lineage and stage of differentiation
Abstract:	Mouse monoclonal antibodies have been used to study the distribution of the epidermal growth factor receptor in human cultured cells and tissues. As expected, most epithelial cells expressed epidermal growth factor receptor (EGFr), whereas cells of hematopoietic origin were EGFr-. However, EGFr was found to be differentially expressed on cultured cells of neuroectodermal origin. Normal melanocytes and a proportion of melanomas are EGFr-, whereas a distinct subset of other melanomas, astrocytomas, and neuroblastomas is EGFr+. Expression of the EGFr in melanomas was closely related to the expression of phenotypic traits of differentiation. Less differentiated melanomas have an epithelioid morphology and are nonpigmented, la+, and EGFr+; in contrast, more differentiated melanomas have a dendritic morphology and are pigmented, la-, and EGFr-. In the melanoma cell panel used, expression of EGFr did not correlate with rate of proliferation. Expression of EGFr in tissues also showed a lack of correlation with the proliferative state of cells. Our findings indicate that expression of EGFr is related to cell lineage and specific stages of cellular differentiation, rather than only to cell proliferation. The data suggest that receptor content may be elevated in a large number of tumor cell lines. © 1986, American Association for Cancer Research. All rights reserved.
Keywords:	human cell; neoplasms; cell proliferation; cytology; cells, cultured; melanoma; melanocyte; epidermal growth factor receptor; cell differentiation; receptor, epidermal growth factor; monoclonal antibody; antibodies, monoclonal; neuroblastoma; tumor cell line; astrocytoma; molecular weight; differentiation; receptors, cell surface; nervous system; desmosome; humans; human; priority journal
Journal Title:	Cancer Research
Volume:	46
Issue:	9
ISSN:	0008-5472
Publisher:	American Association for Cancer Research
Date Published:	1986-09-01
Start Page:	4726
End Page:	4731
Language:	English
PUBMED:	3015394
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022452615&partnerID=40&md5=d0d3cd3a03bb0924c55b6232b8da6367
Notes:	Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: The authors wish to acknowledge Dr. A. N. Houghton for valuable contributions. Dr. N. Dracopoli for valuable discussions, and Dr. H. F. Oettgen for continued support.

https://synapse.mskcc.org/synapse/works/has_related_data_chk/185447