CHD8 associates with human staf and contributes to efficient U6 RNA polymerase III transcription Journal Article
| Authors: | Yuan, C. C.; Zhao, X.; Florens, L.; Swanson, S. K.; Washburn, M. P.; Hernandez, N. |
| Article Title: | CHD8 associates with human staf and contributes to efficient U6 RNA polymerase III transcription |
| Abstract: | Chromatin remodeling and histone modification are essential for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (Pol III)-transcribed genes. The human U6 small nuclear RNA promoter, located 5′ of the transcription start site, consists of a core region directing basal transcription and an activating region that recruits the transcription factors Oct-1 and Staf (ZNF143). Oct-1 activates transcription in part by helping recruit core binding factors, but nothing is known about the mechanisms of transcription activation by Staf. We show that Staf activates U6 transcription from a preassembled chromatin template in vitro and associates with several proteins linked to chromatin modification, among them chromodomain-helicase-DNA binding protein 8 (CHD8). CHD8 binds to histone H3 di-and trimethylated on lysine 4. It resides on the human U6 promoter as well as the mRNA IRF3 promoter in vivo and contributes to efficient transcription from both these promoters. Thus, Pol III transcription from type 3 promoters uses some of the same factors used for chromatin remodeling at Pol II promoters. Copyright © 2007, American Society for Microbiology. All Rights Reserved. |
| Keywords: | controlled study; protein expression; unclassified drug; dna binding protein; human cell; methylation; dna-binding proteins; protein domain; transcription initiation; protein binding; transcription factor; genetic transcription; transcription, genetic; hela cells; transcription factors; amino acid sequence; molecular sequence data; messenger rna; eukaryota; chromatin; nucleotide sequence; histone h3; protein transport; immunoprecipitation; trans-activators; histones; lysine; gene location; chromatin assembly and disassembly; protein modification; core binding factor; interferon regulatory factor 3; trans-activation (genetics); rna, small nuclear; chromodomain helicase dna binding protein 8; dna directed rna polymerase iii; lysine derivative; octamer transcription factor 1; transcription factor staf; interferon regulatory factor-3; rna polymerase iii |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 27 |
| Issue: | 24 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2007-12-01 |
| Start Page: | 8729 |
| End Page: | 8738 |
| Language: | English |
| DOI: | 10.1128/mcb.00846-07 |
| PUBMED: | 17938208 |
| PROVIDER: | scopus |
| PMCID: | PMC2169411 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 14" - "Export Date: 17 November 2011" - "CODEN: MCEBD" - "Molecular Sequence Numbers: GENBANK: NP_065971, XP_370738, XP_509818, XP_573762, XP_619244;" - "Source: Scopus" |
