Human tumor cells segregate into radiosensitivity groups that associate with ATM and TP53 status Journal Article
| Authors: | Williams, J. R.; Zhang, Y.; Russell, J.; Koch, C.; Little, J. B. |
| Article Title: | Human tumor cells segregate into radiosensitivity groups that associate with ATM and TP53 status |
| Abstract: | We seek to determine whether cellular radiosensitivity in nineteen human colorectal tumor cell lines and three human glioblastoma tumor cell lines segregate into statistically distinct groups and whether such groups correlate with gene expression. We measure clonogenic survival in 22 cell lines that vary in radiosensitivity and in expression of selected genes: ATM, TP53, CDKN1A, 14-3-3σ, Ki-ras and DNA mismatch repair genes. We describe and compare radiosensitivity in these cell lines by one-parameter or two parameter analysis. Radiosensitivity varies among and between colorectal tumor cell lines and glioblastoma cell lines. When compared directly using survival, or using two-parameter analysis of radiosensitivity, cell lines distribute into four statistically-significant radiosensitivity groups. These groups associate strongly with the status of two genes, ATM and TP53, but do not associate with CDKN1A, 14-3-3σ, Ki-ras and DNA mismatch repair genes. Intrinsic cellular radiosensitivity of 22 colorectal and glioblastoma cell lines fall into four radiosensitivity groups that associate with expression of ATM and TP53. These analyses suggest multiple mechanisms underlay intrinsic cellular radiosensitivity. © 2007 Taylor & Francis. |
| Keywords: | controlled study; human cell; dna-binding proteins; comparative study; cell cycle proteins; cell survival; gene expression; neoplasm proteins; tumor markers, biological; cancer cell culture; cell line, tumor; protein p53; protein kinase inhibitors; colorectal neoplasms; statistical significance; dna; protein-serine-threonine kinases; colorectal tumor; mismatch repair; dna mismatch repair; glioblastoma; tumor suppressor proteins; tumor cell; atm protein; tumor suppressor protein p53; radiosensitivity; cyclin dependent kinase inhibitor 1a; cyclin-dependent kinase inhibitor p21; genes, ras; k ras protein; cell activity; exonucleases; clonogenesis; radiation tolerance; radiological parameters; tumor stem cell assay; protein 14 3 3 |
| Journal Title: | Acta Oncologica |
| Volume: | 46 |
| Issue: | 5 |
| ISSN: | 0284-186X |
| Publisher: | Informa Healthcare |
| Date Published: | 2007-01-01 |
| Start Page: | 628 |
| End Page: | 638 |
| Language: | English |
| DOI: | 10.1080/02841860601080407 |
| PUBMED: | 17562439 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 10" - "Export Date: 17 November 2011" - "CODEN: ACTOE" - "Source: Scopus" |
