L3MBTL1, a histone-methylation-dependent chromatin lock Journal Article
| Authors: | Trojer, P.; Li, G.; Sims, R. J. 3rd; Vaquero, A.; Kalakonda, N.; Boccuni, P.; Lee, D.; Erdjument-Bromage, H.; Tempst, P.; Nimer, S. D.; Wang, Y. H.; Reinberg, D. |
| Article Title: | L3MBTL1, a histone-methylation-dependent chromatin lock |
| Abstract: | Distinct histone lysine methylation marks are involved in transcriptional repression linked to the formation and maintenance of facultative heterochromatin, although the underlying mechanisms remain unclear. We demonstrate that the malignant-brain-tumor (MBT) protein L3MBTL1 is in a complex with core histones, histone H1b, HP1γ, and Rb. The MBT domain is structurally related to protein domains that directly bind methylated histone residues. Consistent with this, we found that the L3MBTL1 MBT domains compact nucleosomal arrays dependent on mono- and dimethylation of histone H4 lysine 20 and of histone H1b lysine 26. The MBT domains bind at least two nucleosomes simultaneously, linking repression of transcription to recognition of different histone marks by L3MBTL1. Consistently, L3MBTL1 was found to negatively regulate the expression of a subset of genes regulated by E2F, a factor that interacts with Rb. © 2007 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; unclassified drug; human cell; methylation; protein domain; complex formation; gene expression; signaling; neoplasm proteins; cell line; protein binding; protein interaction; hela cells; dna; histone; chromatin; chromatin immunoprecipitation; histone h3; tumor protein; retinoblastoma protein; histones; nucleosome; nucleosomes; histone h4; heterochromatin protein 1; protein l3mbtl1; heterochromatin protein 1gamma; histone h1; histone h1b; e2f transcription factors |
| Journal Title: | Cell |
| Volume: | 129 |
| Issue: | 5 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2007-06-01 |
| Start Page: | 915 |
| End Page: | 928 |
| Language: | English |
| DOI: | 10.1016/j.cell.2007.03.048 |
| PUBMED: | 17540172 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 106" - "Export Date: 17 November 2011" - "CODEN: CELLB" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work