Synthesis and characterization of azido aryl analogs of IBNtxA for radio-photoaffinity labeling opioid receptors in cell lines and in mouse brain Journal Article
| Authors: | Grinnell, S. G.; Uprety, R.; Varadi, A.; Subrath, J.; Hunkele, A.; Pan, Y. X.; Pasternak, G. W.; Majumdar, S. |
| Article Title: | Synthesis and characterization of azido aryl analogs of IBNtxA for radio-photoaffinity labeling opioid receptors in cell lines and in mouse brain |
| Abstract: | Mu opioid receptors (MOR-1) mediate the biological actions of clinically used opioids such as morphine, oxycodone, and fentanyl. The mu opioid receptor gene, OPRM1, undergoes extensive alternative splicing, generating multiple splice variants. One type of splice variants are truncated variants containing only six transmembrane domains (6TM) that mediate the analgesic action of novel opioid drugs such as 3′-iodobenzoylnaltrexamide (IBNtxA). Previously, we have shown that IBNtxA is a potent analgesic effective in a spectrum of pain models but lacks many side-effects associated with traditional opiates. In order to investigate the targets labeled by IBNtxA, we synthesized two arylazido analogs of IBNtxA that allow photolabeling of mouse mu opioid receptors (mMOR-1) in transfected cell lines and mMOR-1 protein complexes that may comprise the 6TM sites in mouse brain. We demonstrate that both allyl and alkyne arylazido derivatives of IBNtxA efficiently radio-photolabeled mMOR-1 in cell lines and MOR-1 protein complexes expressed either exogenously or endogenously, as well as found in mouse brain. In future, design and application of such radio-photolabeling ligands with a conjugated handle will provide useful tools for further isolating or purifying MOR-1 to investigate site specific ligand–protein contacts and its signaling complexes. © 2020, Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | opioid; photoaffinity labeling; click; ibntxa; radioligand binding; mu opioid receptor complex |
| Journal Title: | Cellular and Molecular Neurobiology |
| Volume: | 41 |
| Issue: | 5 |
| ISSN: | 0272-4340 |
| Publisher: | Springer |
| Date Published: | 2021-07-01 |
| Start Page: | 977 |
| End Page: | 993 |
| Language: | English |
| DOI: | 10.1007/s10571-020-00867-6 |
| PUBMED: | 32424771 |
| PROVIDER: | scopus |
| PMCID: | PMC7671950 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 July 2021 -- Source: Scopus |
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