Endogenous p53 expression in human and mouse is not regulated by its 30'UTR Journal Article
| Authors: | Mitschka, S.; Mayr, C. |
| Article Title: | Endogenous p53 expression in human and mouse is not regulated by its 30'UTR |
| Abstract: | The TP53 gene encodes the tumor suppressor p53 which is functionally inactivated in many human cancers. Numerous studies suggested that 30UTR-mediated p53 expression regulation plays a role in tumorigenesis and could be exploited for therapeutic purposes. However, these studies did not investigate post-transcriptional regulation of the native TP53 gene. Here, we used CRISPR/Cas9 to delete the human and mouse TP53/Trp53 30UTRs while preserving endogenous mRNA processing. This revealed that the endogenous 30UTR is not involved in regulating p53 mRNA or protein expression neither in steady state nor after genotoxic stress. Using reporter assays, we confirmed the previously observed repressive effects of the isolated 30UTR. However, addition of the TP53 coding region to the reporter had a dominant negative impact on expression as its repressive effect was stronger and abrogated the contribution of the 30UTR. Our data highlight the importance of genetic models in the validation of post-transcriptional gene regulatory effects. © Mitschka and Mayr. |
| Journal Title: | eLife |
| Volume: | 10 |
| ISSN: | 2050-084X |
| Publisher: | eLife Sciences Publications Ltd. |
| Date Published: | 2021-05-06 |
| Start Page: | e65700 |
| Language: | English |
| DOI: | 10.7554/eLife.65700 |
| PUBMED: | 33955355 |
| PROVIDER: | scopus |
| PMCID: | PMC8137139 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 July 2021 -- Source: Scopus |
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