Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9 Journal Article

   


Authors: Zhong, A.; Li, M.; Zhou, T.
Article Title: Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9
Abstract: Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made this effort an arduous process in hPSCs. Here, we provide a step-by-step protocol and practical guide for generating reporter lines in hPSCs via CRISPR/Cas9-mediated HDR. We also elaborate on the process of generating a TBXT-GFP reporter line as an example. © 2020 The Author(s)
Journal Title: STAR Protocols
Volume: 1
Issue: 2
ISSN: 2666-1667
Publisher: Cell Press  
Date Published: 2020-01-01
Start Page: 100052
Language: English
DOI: 10.1016/j.xpro.2020.100052
PROVIDER: scopus
PMCID: PMC7566848
PUBMED: 33073252
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108283531&doi=10.1016%2fj.xpro.2020.100052&partnerID=40&md5=ead48e84d541a4db9c784b7bb2c59756
Notes: Article -- Export Date: 1 July 2021 -- Source: Scopus
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MSK Authors
  1. Ming Li
    110 Li
  2. Ting Zhou
    26 Zhou
  3. Aaron Zhong
    9 Zhong
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