Structure of 3-PO(4)/5-OH RNA ligase RtcB in complex with a 5-OH oligonucleotide Journal Article
| Authors: | Banerjee, A.; Goldgur, Y.; Shuman, S. |
| Article Title: | Structure of 3-PO(4)/5-OH RNA ligase RtcB in complex with a 5-OH oligonucleotide |
| Abstract: | RtcB enzymes comprise a widely distributed family of manganese-and GTP-dependent RNA repair enzymes that join 2,3-cyclic phosphate ends to 5-OH ends via RtcB-(histidinyl-N) GMP, RNA 3-phosphate, and RNA3pp5G intermediates. RtcB can ligate either 5-OH RNA or 5-OH DNA strands in vitro. The nucleic acid contacts of RtcB are uncharted. Here we report a 2.7 A crystal structure of Pyrococcus horikoshii RtcB in complex with a 6-mer 5-OH DNA oligonucleotide HOA1pT2pG3pT4pC5pC6, which reveals enzymic contacts of Asn202 to the terminal 5-OH nucleophile; Arg238 to the A1pT2 and T2pG3 phosphates; Arg190 and Gln194 to the T2pG3 phosphate; and an Arg190-cation interaction with the G3 nucleobase. The structural insights affirm functional studies of E. coli RtcB that implicated the conserved counterpart of Arg238 in engagement of the 5-OH strand for ligation. The essential active site Cys98 that coordinates two manganese ions is oxidized to cysteine sulfonic acid in our structure, raising the prospect that RtcB activity might be sensitive to modulation during oxidative stress. © 2021 Cold Spring Harbor Laboratory Press. All rights reserved. |
| Keywords: | trna splicing; rna repair; cysteine sulfonic acid |
| Journal Title: | RNA |
| Volume: | 27 |
| Issue: | 5 |
| ISSN: | 1355-8382 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2021-05-01 |
| Start Page: | 584 |
| End Page: | 590 |
| Language: | English |
| DOI: | 10.1261/rna.078692.121 |
| PUBMED: | 33619169 |
| PROVIDER: | scopus |
| PMCID: | PMC8051266 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2021 -- Source: Scopus |
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