Metastasis and immune evasion from extracellular cGAMP hydrolysis Journal Article
| Authors: | Li, J.; Duran, M. A.; Dhanota, N.; Chatila, W. K.; Bettigole, S. E.; Kwon, J.; Sriram, R. K.; Humphries, M. P.; Salto-Tellez, M.; James, J. A.; Hanna, M. G.; Melms, J. C.; Vallabhaneni, S.; Litchfield, K.; Usaite, I.; Biswas, D.; Bareja, R.; Li, H. W.; Martin, M. L.; Dorsaint, P.; Cavallo, J. A.; Li, P.; Pauli, C.; Gottesdiener, L.; Dipardo, B. J.; Hollmann, T. J.; Merghoub, T.; Wen, H. Y.; Reis-Filho, J. S.; Riaz, N.; Su, S. S. M.; Kalbasi, A.; Vasan, N.; Powell, S. N.; Wolchok, J. D.; Elemento, O.; Swanton, C.; Shoushtari, A. N.; Parkes, E. E.; Izar, B.; Bakhoum, S. F. |
| Article Title: | Metastasis and immune evasion from extracellular cGAMP hydrolysis |
| Abstract: | Cytosolic DNA is characteristic of chromosomally unstable metastatic cancer cells, resulting in constitutive activation of the cGAS–STING innate immune pathway. How tumors co-opt inflammatory signaling while evading immune surveillance remains unknown. Here, we show that the ectonucleotidase ENPP1 promotes metastasis by selectively degrading extracellular cGAMP, an immune-stimulatory metabolite whose breakdown products include the immune suppressor adenosine. ENPP1 loss suppresses metastasis, restores tumor immune infiltration, and potentiates response to immune checkpoint blockade in a manner dependent on tumor cGAS and host STING. Conversely, overexpression of wild-type ENPP1, but not an enzymatically weakened mutant, promotes migration and metastasis, in part through the generation of extracellular adenosine, and renders otherwise sensitive tumors completely resistant to immunotherapy. In human cancers, ENPP1 expression correlates with reduced immune cell infiltration, increased metastasis, and resistance to anti–PD-1/ PD-L1 treatment. Thus, cGAMP hydrolysis by ENPP1 enables chromosomally unstable tumors to trans-mute cGAS activation into an immune-suppressive pathway. Significance: Chromosomal instability promotes metastasis by generating chronic tumor inflamma-tion. ENPP1 facilitates metastasis and enables tumor cells to tolerate inflammation by hydrolyzing the immunotransmitter cGAMP, preventing its transfer from cancer cells to immune cells. © 2020 American Association for Cancer Research. |
| Journal Title: | Cancer Discovery |
| Volume: | 11 |
| Issue: | 5 |
| ISSN: | 2159-8274 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2021-05-01 |
| Start Page: | 1212 |
| End Page: | 1227 |
| Language: | English |
| DOI: | 10.1158/2159-8290.Cd-20-0387 |
| PUBMED: | 33372007 |
| PROVIDER: | scopus |
| PMCID: | PMC8102348 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2021 -- Source: Scopus |
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