Analysis of integrin signaling in genetically engineered mouse models of mammary tumor progression Journal Article
| Authors: | Pylayeva, Y.; Guo, W.; Giancotti, F. G. |
| Article Title: | Analysis of integrin signaling in genetically engineered mouse models of mammary tumor progression |
| Abstract: | Cancer progression-the evolution of malignant tumors towards metastatic dissemination-is a complex, multistep process orchestrated by neoplastic cells but aided by elements of the tumor microenvironment such as macrophages, activated fibroblasts, and endothelial cells. During tumor progression, cancer cells acquire a number of traits, such as the ability to undergo unrestrained proliferation, to resist pro-apoptotic insults, and to invade through tissue boundaries. Genetic and epigenetic changes conspire to drive the emergence of these traits against the backdrop of host selection. It is becoming increasingly clear that certain integrins and integrin-signaling components amplify oncogenic signaling to promote tumor progression. Mouse models of cancer provide useful, if not necessary, experimental systems to study tumor initiation and progression in vivo and to test novel therapeutic approaches. We have utilized mouse models of mammary tumorigenesis to examine the role of integrin α6β4 signaling in tumor progression in vivo. In this chapter, we describe a collection of cell biological and genetic methods that may aid in characterizing the roles of integrin signals in mammary tumorigenesis. © 2007 Elsevier Inc. All rights reserved. |
| Keywords: | immunohistochemistry; signal transduction; controlled study; genetics; disease course; review; nonhuman; methodology; cell proliferation; mouse; animal; animals; mice; animal tissue; apoptosis; gene amplification; animal experiment; animal model; in vivo study; pathology; cancer model; physiology; transgenic mouse; cancer invasion; endothelium cell; disease model; oncogene; genetic engineering; epigenetics; disease progression; breast tumor; cancer cell; neoplasms, experimental; fibroblast; malignant neoplastic disease; microenvironment; experimental neoplasm; tumor growth; disease models, animal; macrophage; cell activation; breast carcinogenesis; integrin; integrins; mammary neoplasms, animal; alpha6beta4 integrin; host selection |
| Journal Title: | Methods in Enzymology |
| Volume: | 426 |
| ISSN: | 0076-6879 |
| Publisher: | Academic Press |
| Publication Place: | San Diego, CA |
| Date Published: | 2007-01-01 |
| Start Page: | 439 |
| End Page: | 461 |
| Language: | English |
| DOI: | 10.1016/s0076-6879(07)26019-7 |
| PUBMED: | 17697895 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Chapter 19 in "Integrins" (ISBN: 978-0-12-373924-7) --- - Cited By (since 1996): 2 - Export Date: 17 November 2011 - CODEN: MENZA - Source: Scopus |
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