The AIM2 inflammasome exacerbates atherosclerosis in clonal haematopoiesis Journal Article

Authors: Fidler, T. P.; Xue, C.; Yalcinkaya, M.; Hardaway, B.; Abramowicz, S.; Xiao, T.; Liu, W.; Thomas, D. G.; Hajebrahimi, M. A.; Pircher, J.; Silvestre-Roig, C.; Kotini, A. G.; Luchsinger, L. L.; Wei, Y.; Westerterp, M.; Snoeck, H. W.; Papapetrou, E. P.; Schulz, C.; Massberg, S.; Soehnlein, O.; Ebert, B.; Levine, R. L.; Reilly, M. P.; Libby, P.; Wang, N.; Tall, A. R.
Article Title: The AIM2 inflammasome exacerbates atherosclerosis in clonal haematopoiesis
Abstract: Clonal haematopoiesis, which is highly prevalent in older individuals, arises from somatic mutations that endow a proliferative advantage to haematopoietic cells. Clonal haematopoiesis increases the risk of myocardial infarction and stroke independently of traditional risk factors1. Among the common genetic variants that give rise to clonal haematopoiesis, the JAK2V617F (JAK2VF) mutation, which increases JAK–STAT signalling, occurs at a younger age and imparts the strongest risk of premature coronary heart disease1,2. Here we show increased proliferation of macrophages and prominent formation of necrotic cores in atherosclerotic lesions in mice that express Jak2VF selectively in macrophages, and in chimeric mice that model clonal haematopoiesis. Deletion of the essential inflammasome components caspase 1 and 11, or of the pyroptosis executioner gasdermin D, reversed these adverse changes. Jak2VF lesions showed increased expression of AIM2, oxidative DNA damage and DNA replication stress, and Aim2 deficiency reduced atherosclerosis. Single-cell RNA sequencing analysis of Jak2VF lesions revealed a landscape that was enriched for inflammatory myeloid cells, which were suppressed by deletion of Gsdmd. Inhibition of the inflammasome product interleukin-1β reduced macrophage proliferation and necrotic formation while increasing the thickness of fibrous caps, indicating that it stabilized plaques. Our findings suggest that increased proliferation and glycolytic metabolism in Jak2VF macrophages lead to DNA replication stress and activation of the AIM2 inflammasome, thereby aggravating atherosclerosis. Precise application of therapies that target interleukin-1β or specific inflammasomes according to clonal haematopoiesis status could substantially reduce cardiovascular risk. © 2021, The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature.
Keywords: mutation; metabolism; genetic variation; risk assessment; immune response; rodent; oxidation; damage; cell component
Journal Title: Nature
Volume: 592
Issue: 7853
ISSN: 0028-0836
Publisher: Nature Publishing Group  
Date Published: 2021-04-08
Start Page: 296
End Page: 301
Language: English
DOI: 10.1038/s41586-021-03341-5
PUBMED: 33731931
PROVIDER: scopus
PMCID: PMC8038646
Notes: Article -- Export Date: 3 May 2021 -- Source: Scopus
Citation Impact
MSK Authors
  1. Ross Levine
    606 Levine