Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes Journal Article
| Authors: | Palancade, B.; Liu, X.; Garcia-Rubio, M.; Aguilera, A.; Zhao, X.; Doye, V. |
| Article Title: | Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes |
| Abstract: | Increasing evidences suggest that nuclear pore complexes (NPCs) control different aspects of nuclear metabolism, including transcription, nuclear organization, and DNA repair. We previously established that the Nup84 complex, a major NPC building block, is part of a genetic network involved in DNA repair. Here, we show that double-strand break (DSB) appearance is linked to a shared function of the Nup84 and the Nup60/Mlp1-2 complexes. Mutants within these complexes exhibit similar genetic interactions and alteration in DNA repair processes as mutants of the SUMO-protease Ulp1. Consistently, these nucleoporins are required for maintenance of proper Ulp1 levels at NPCs and for the establishment of the appropriate sumoylation of several cellular proteins, including the DNA repair factor Yku70. Moreover, restoration of nuclear envelope-associated Ulp1 in nucleoporin mutants reestablishes proper sumoylation patterns and suppresses DSB accumulation and genetic interactions with DNA repair genes. Our results thus provide a molecular mechanism that underlies the connection between NPC and genome stability. © 2007 by The American Society for Cell Biology. |
| Keywords: | unclassified drug; mutation; nonhuman; protein function; protein localization; cell nucleus membrane; phenotype; cytology; dna damage; dna repair; cell protein; protein; genetic transcription; nuclear proteins; saccharomyces cerevisiae; genomic instability; gene interaction; protein transport; dna breaks, double-stranded; saccharomyces cerevisiae proteins; sumo protein; sumoylation; small ubiquitin-related modifier proteins; nuclear pore complex proteins; enzyme stability; karyopherins; nucleoporin; cysteine endopeptidases; nuclear pore complex; nuclear envelope; nucleoporin 60; nucleoporin 84; protein mlp1; protein mlp2; protein ulp1; protein yku70 |
| Journal Title: | Molecular Biology of the Cell |
| Volume: | 18 |
| Issue: | 8 |
| ISSN: | 1059-1524 |
| Publisher: | The American Society for Cell Biology |
| Date Published: | 2007-08-01 |
| Start Page: | 2912 |
| End Page: | 2923 |
| Language: | English |
| DOI: | 10.1091/mbc.E07-02-0123 |
| PUBMED: | 17538013 |
| PROVIDER: | scopus |
| PMCID: | PMC1949349 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 26" - "Export Date: 17 November 2011" - "CODEN: MBCEE" - "Source: Scopus" |
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