Telomere length, telomerase activity, and expressions of human telomerase mRNA component (hTERC) and human telomerase reverse transcriptase (hTERT) mRNA in pulmonary neuroendocrine tumors Journal Article
| Authors: | Nishio, Y.; Nakanishi, K.; Ozeki, Y.; Jiang, S. X.; Kameya, T.; Hebisawa, A.; Mukai, M.; Travis, W. D.; Franks, T. J.; Kawai, T. |
| Article Title: | Telomere length, telomerase activity, and expressions of human telomerase mRNA component (hTERC) and human telomerase reverse transcriptase (hTERT) mRNA in pulmonary neuroendocrine tumors |
| Abstract: | Background: Telomeres are important for chromosome structure and function, protecting them against degradation. However, few studies have examined telomeres in pulmonary neuroendocrine (NE) tumors. Methods: We investigated deparaffinized sections obtained from 70 primary NE lung tumors [34 typical carcinoids (TCs), 10 atypical carcinoids (ACs), 16 large cell neuroendocrine carcinoma (LCNECs) and 10 small cell lung carcinomas (SCLCs)]. Results: Positive expressions of human telomerase mRNA component (hTERC) and human telomerase reverse transcriptase (hTERT) mRNA were recognized, respectively, in 58% and 74% of TCs, and in 100%and 100% of ACs, LCNECs and SCLCs. Alteration of telomere length was greater in both LCNECs and SCLCs than in TCs. Telomerase activity was detected in LCNECs, but not in TCs. By the reverse-transcriptase polymerase chain reaction (RT-PCR), hTERC mRNA was detected in 100% of LCNECs and TCs examined, while hTERT mRNA was detected in 67% of LCNECs, but not at all in TCs. Conclusions: These results suggest that alterations in telomere length, telomerase activity, and the expression of hTERT mRNA may (i) play roles in pathogenesis in pulmonary neuroendocrine tumors, and (ii) be a useful tool for differential diagnosis between TCs and LCNECs. © 2006 Foundation for Promotion of Cancer Research. |
| Keywords: | controlled study; human tissue; telomere; reverse transcription polymerase chain reaction; gene expression; lung neoplasms; differential diagnosis; enzyme activity; telomerase; neuroendocrine tumor; lung tumor; rna; in situ hybridization; lung small cell cancer; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; telomerase reverse transcriptase; carcinoid; large cell carcinoma; lung carcinogenesis; neuroendocrine tumors; carcinoma, neuroendocrine; human telomerase mrna component (hterc); telomerase reverse transcriptase (htert) |
| Journal Title: | Japanese Journal of Clinical Oncology |
| Volume: | 37 |
| Issue: | 1 |
| ISSN: | 0368-2811 |
| Publisher: | Oxford University Press |
| Date Published: | 2007-01-01 |
| Start Page: | 16 |
| End Page: | 22 |
| Language: | English |
| DOI: | 10.1093/jjco/hyl118 |
| PUBMED: | 17060405 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 3" - "Export Date: 17 November 2011" - "CODEN: JJCOA" - "Source: Scopus" |
